The effect of anthocyans on the expression of selected phase II xenobiotic-metabolizing enzymes in primary cultures of human hepatocytes
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25008252
DOI
10.1039/c4fo00347k
Knihovny.cz E-resources
- MeSH
- Anthocyanins pharmacology MeSH
- Glucuronosyltransferase genetics metabolism MeSH
- Hepatocytes drug effects enzymology metabolism MeSH
- Metabolic Detoxication, Phase II * MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Xenobiotics metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anthocyanins MeSH
- Glucuronosyltransferase MeSH
- Xenobiotics MeSH
Anthocyans are biologically active constituents of various berry fruits and they are also contained in nutritional supplements derived from extracts or dry matter from berry fruits. In this study we evaluated the effects of anthocyans on the expression of selected drug-metabolizing phase II genes in primary cultures of human hepatocytes by qRT-PCR. Most of the tested anthocyanidins (6) and anthocyanins (21) did not induce the expression of mRNA of UGT1A/2B members in human hepatocytes. The same can be stated for expression of selected GST genes on the mRNA level. However, some of them e.g. cyanidin-3-O-rutinoside consistently decreased the level of GSTP1 mRNA in all tested cultures. In conclusion, most of the anthocyans did not affect the expression of selected phase II metabolizing enzymes in vitro.
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