The variation in the diversity of methanogens in sediment depths from Sitka stream was studied by constructing a 16S rRNA gene library using methanogen-specific primers and a denaturing gradient gel electrophoresis (DGGE)-based approach. A total of nine different phylotypes from the 16S rRNA library were obtained, and all of them were clustered within the order Methanosarcinales. These nine phylotypes likely represent nine new species and at least 5-6 new genera. Similarly, DGGE analysis revealed an increase in the diversity of methanogens with an increase in sediment depth. These results suggest that Methanosarcinales phylotypes might be the dominant methanogens in the sediment from Sitka stream, and the diversity of methanogens increases as the depth increases. Results of the present study will help in making effective strategies to monitor the dominant methanogen phylotypes and methane emissions in the environment.

Laboratory of Aquatic Microbial Ecology Department of Ecology and Environmental Sciences Faculty of Science Palacky University Šlechtitelů 11 783 71 Olomouc Czech Republic

Zobrazit více v PubMed

Appl Environ Microbiol. 2007 Jul;73(13):4206-10 PubMed

Appl Environ Microbiol. 2009 Dec;75(23):7537-41 PubMed

Mol Biol Evol. 1995 Sep;12(5):823-33 PubMed

Genome Res. 2011 Mar;21(3):494-504 PubMed

Appl Environ Microbiol. 1998 Dec;64(12):4983-9 PubMed

Microb Ecol. 2007 Nov;54(4):697-704 PubMed

Syst Appl Microbiol. 2007 Apr;30(3):239-54 PubMed

Environ Microbiol Rep. 2009 Oct;1(5):285-92 PubMed

Gene. 2012 Feb 1;493(1):13-7 PubMed

Microb Ecol. 2012 Jul;64(1):131-9 PubMed

Antonie Van Leeuwenhoek. 2011 May;99(4):739-51 PubMed

Appl Microbiol Biotechnol. 2013 Sep;97(17):7553-62 PubMed

Appl Environ Microbiol. 1997 Dec;63(12):4729-33 PubMed

Environ Microbiol. 2005 Aug;7(8):1139-49 PubMed

Proc Natl Acad Sci U S A. 2004 Jul 27;101(30):11030-5 PubMed

J Mol Evol. 1980 Dec;16(2):111-20 PubMed

Microb Ecol. 2008 Aug;56(2):390-4 PubMed

FEMS Microbiol Ecol. 2009 Oct;70(1):87-98 PubMed

Environ Microbiol. 2011 Aug;13(8):2336-41 PubMed

Microb Ecol. 2011 Jul;62(1):1-13 PubMed

Appl Environ Microbiol. 2005 Aug;71(8):4592-601 PubMed

Anaerobe. 2000 Aug;6(4):205-26 PubMed

Environ Microbiol. 2006 Apr;8(4):709-19 PubMed

Mol Biol Evol. 1987 Jul;4(4):406-25 PubMed

Lett Appl Microbiol. 2009 Aug;49(2):274-7 PubMed

Mol Biol Evol. 2011 Oct;28(10):2731-9 PubMed

J Microbiol Methods. 2003 Nov;55(2):337-49 PubMed

J Environ Sci (China). 2008;20(2):224-30 PubMed

FEMS Microbiol Lett. 2004 Mar 19;232(2):153-63 PubMed

PLoS One. 2013 Nov 20;8(11):e80804 PubMed

FEMS Microbiol Ecol. 2012 Jul;81(1):243-54 PubMed

Appl Environ Microbiol. 2006 Feb;72(2):1663-6 PubMed

Environ Microbiol. 2004 Mar;6(3):274-87 PubMed

FEMS Microbiol Ecol. 2011 Sep;77(3):533-45 PubMed

FEMS Microbiol Ecol. 2002 Jan 1;39(1):17-21 PubMed

Evolution. 1985 Jul;39(4):783-791 PubMed

Methods Enzymol. 1996;266:131-41 PubMed

Environ Microbiol. 2009 Mar;11(3):657-68 PubMed

Lett Appl Microbiol. 2009 Mar;48(3):386 PubMed

Geobiology. 2008 Aug;6(4):376-93 PubMed

Is the methanogenic community reflecting the methane emissions of river sediments?-comparison of two study sites