Differential expression of tripartite motif-containing family in normal human dermal fibroblasts in response to porcine endogenous retrovirus infection
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25056437
DOI
10.14712/fb2014060030144
PII: file/5726/fb2014a0018.pdf
Knihovny.cz E-zdroje
- MeSH
- aminokyselinové motivy MeSH
- endogenní retroviry fyziologie MeSH
- fibroblasty metabolismus virologie MeSH
- lidé MeSH
- messenger RNA genetika metabolismus MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- reprodukovatelnost výsledků MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
- škára patologie MeSH
- stanovení celkové genové exprese * MeSH
- Sus scrofa MeSH
- transportní proteiny genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- messenger RNA MeSH
- transportní proteiny MeSH
Antiretroviral restriction factors may play an essential role in the safety of xenotransplantation. Therefore, the present study focused on investigation of the changes in the tripartite motif-containing family (TRIM) gene expression in normal human dermal fibroblasts with and without lipopolysaccharide stimulation in response to porcine endogenous retrovirus infection. Analysis of the expression profile of TRIMs was performed using oligonucleotide microarrays and QRT-PCR. Nine (TRIM1, TRIM2, TRIM5, TRIM14, TRIM16, TRIM18, TRIM22, TRIM27 and TRIM31) statistically significantly differentially expressed genes were found (P < 0.05, one-way ANOVA). In conclusion, comprehensive analysis of retroviral restriction factor gene expression in human dermal fibroblasts before and after porcine endogenous retrovirus infection with and without LPS stimulation may suggest association of the selected TRIMs with antiretroviral activity.
Department of Food and Nutrition Medical University of Silesia Sosnowiec Poland
Department of Molecular Biology Medical University of Silesia Sosnowiec Poland
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