Integrative chemical proteomics and cell biology methods to study endocytosis and vesicular trafficking in Arabidopsis
Language English Country United States Media print
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
Grant support
P20 GM103476
NIGMS NIH HHS - United States
P20GM103476
NIGMS NIH HHS - United States
- MeSH
- Androstadienes administration & dosage MeSH
- Arabidopsis drug effects MeSH
- Brefeldin A administration & dosage MeSH
- Endocytosis drug effects MeSH
- Molecular Biology methods MeSH
- Arabidopsis Proteins biosynthesis MeSH
- Proteomics * MeSH
- Transport Vesicles drug effects MeSH
- Vesicular Transport Proteins biosynthesis MeSH
- Wortmannin MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- Androstadienes MeSH
- Brefeldin A MeSH
- Arabidopsis Proteins MeSH
- Vesicular Transport Proteins MeSH
- Wortmannin MeSH
We present a comprehensive approach combining proteomics and cell biology to study vesicular trafficking in plants. Within this approach, we exploit chemical compounds inhibiting particular vesicular trafficking events in plant cells. Treatment of plants with these relatively specific inhibitors results in intracellular accumulation of proteins being transported by vesicles as well as in a change in abundance of regulatory proteins. Such pharmacological inhibition allows for identification of key proteins, and for further detailed functional investigation using cell biological, molecular biological, and biochemical methods used for validation of proteomic results.
References provided by Crossref.org
Comparative proteomic study of Arabidopsis mutants mpk4 and mpk6
