In plants, membrane compartmentalization requires vesicle trafficking for communication among distinct organelles. Membrane proteins involved in vesicle trafficking are highly dynamic and can respond rapidly to changes in the environment and to cellular signals. Capturing their localization and dynamics is thus essential for understanding the mechanisms underlying vesicular trafficking pathways. Quantitative mass spectrometry and imaging approaches allow a system-wide dissection of the vesicular proteome, the characterization of ligand-receptor pairs and the determination of secretory, endocytic, recycling and vacuolar trafficking pathways. In this review, we highlight major proteomics and imaging methods employed to determine the location, distribution and abundance of proteins within given trafficking routes. We focus in particular on methodologies for the elucidation of vesicle protein dynamics and interactions and their connections to downstream signalling outputs. Finally, we assess their biological applications in exploring different cellular and subcellular processes.

College of Biological Sciences and Technology Beijing Forestry University Beijing China

College of Life Science Henan Normal University Xinxiang China

Department of Biotechnology Faculty of Science Palacky University Olomouc Olomouc Czech Republic

Department of Cell Biology Centre of the Region Hana for Biotechnological and Agricultural Research Faculty of Science Palacky University Olomouc Olomouc Czech Republic

State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing China

Zobrazit více v PubMed

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