Wortmannin treatment induces changes in Arabidopsis root proteome and post-Golgi compartments
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
22524784
DOI
10.1021/pr201111n
Knihovny.cz E-zdroje
- MeSH
- androstadieny farmakologie MeSH
- Arabidopsis cytologie účinky léků metabolismus MeSH
- fosfolipasa D metabolismus MeSH
- kořeny rostlin cytologie účinky léků metabolismus MeSH
- multivezikulární tělíska účinky léků metabolismus MeSH
- proteiny huseníčku metabolismus MeSH
- proteiny přenášející fosfolipidy metabolismus MeSH
- proteom metabolismus MeSH
- Rab proteiny vázající GTP metabolismus MeSH
- trans-Golgiho síť účinky léků metabolismus ultrastruktura MeSH
- transport proteinů účinky léků MeSH
- wortmannin MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- androstadieny MeSH
- fosfolipasa D MeSH
- phospholipase D1 MeSH Prohlížeč
- proteiny huseníčku MeSH
- proteiny přenášející fosfolipidy MeSH
- proteom MeSH
- Rab proteiny vázající GTP MeSH
- wortmannin MeSH
Wortmannin is a widely used pharmaceutical compound which is employed to define vesicular trafficking routes of particular proteins or cellular compounds. It targets phosphatidylinositol 3-kinase and phosphatidylinositol 4-kinases in a dose-dependent manner leading to the inhibition of protein vacuolar sorting and endocytosis. Combined proteomics and cell biological approaches have been used in this study to explore the effects of wortmannin on Arabidopsis root cells, especially on proteome and endomembrane trafficking. On the subcellular level, wortmannin caused clustering, fusion, and swelling of trans-Golgi network (TGN) vesicles and multivesicular bodies (MVBs) leading to the formation of wortmannin-induced multivesicular compartments. Appearance of wortmannin-induced compartments was associated with depletion of TGN as revealed by electron microscopy. On the proteome level, wortmannin induced massive changes in protein abundance profiles. Wortmannin-sensitive proteins belonged to various functional classes. An inhibition of vacuolar trafficking by wortmannin was related to the downregulation of proteins targeted to the vacuole, as showed for vacuolar proteases. A small GTPase, RabA1d, which regulates vesicular trafficking at TGN, was identified as a new protein negatively affected by wortmannin. In addition, Sec14 was upregulated and PLD1 alpha was downregulated by wortmannin.
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