OBJECTIVE: To determine amniotic fluid total antioxidant capacity (TAC), ferric-reducing antioxidant power (FRAP) and thiobarbituric acid-reacting substances (TBARS), markers of oxidative stress, in pregnancies complicated by preterm prelabor rupture of membranes (pPROM) and their correlation to microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA). METHODS: One-hundred thirty-eight women with singleton pregnancies complicated by pPROM were included in this study. Amniotic fluid was collected by transabdominal amniocentesis at the time of admission and amniotic fluid concentrations of TAC, FRAP and TBARS were measured. RESULT: The presence of MIAC and/or HCA did not show any significant differences in the amniotic fluid TAC, FRAP and TBARS concentrations. Positive correlations between gestational age at sampling and amniotic fluid TAC and FRAP concentrations were found (TAC: rho = 0.32; p = 0.0002; FRAP: rho = 0.36; p < 0.0001). A negative correlation between gestation age at sampling and amniotic fluid TBARS concentrations was identified (rho = -0.25; p = 0.004). CONCLUSIONS: Oxidative stress is associated with pPROM as indicated by the presence of markers tested in the amniotic fluid; however, oxidative stress markers tested are not influenced by the presence of MIAC or HCA.

b Department of Obstetrics and Gynecology Faculty of Medicine Hradec Kralove Charles University Prague Prague Czech Republic

Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic

c Institute of Molecular Biomedicine Comenius University in Bratislava Bratislava Slovak Republic

Center for Molecular Medicine Slovak Academy of Sciences Bratislava Slovakia

e Department of Obstetrics and Gynecology The University of Texas Medical Branch at Galveston Galveston USA

f Fingerland's Department of Pathology Faculty of Medicine Hradec Kralove Charles University Prague University Hospital Hradec Kralove Hradec Kralove Czech Republic and

g Department of Obstetrics and Gynecology Palacky University Olomouc University Hospital Olomouc Olomouc Czech Republic

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Maternal white blood cell count cannot identify the presence of microbial invasion of the amniotic cavity or intra-amniotic inflammation in women with preterm prelabor rupture of membranes