Frequent infections with Diplostomum spp. (Digenea: Diplostomidae) were found in the freshwater snail Radix peregra (Müller) and three fish species, the salmonids Salmo trutta fario L., Salvelinus alpinus (L.) and the gasterosteid Gasterosteus aculeatus L., collected in four lakes in south-western Iceland in 2012. Detailed analysis of the isolates integrating molecular, morphological and ecological data revealed that these belong to Diplostomum spathaceum (Rudolphi, 1819) and five putative new species (three infecting both snails and fish). This paper provides detailed descriptions of the metacercariae of the six species-level lineages of Diplostomum spp. and of the cercariae of three of the lineages discovered in Iceland with comments on the application of ITS1 rDNA for species distinction within Diplostomum von Nordmann, 1832 in the light of the novel data. We strongly suggest the use of molecular evidence based on cox1 gene sequences (in addition to ITS1-5.8S-ITS2 sequences) in association with detailed assessment of the morphology of the larval stages in future studies of Diplostomum spp. in fish and snails.

Institute of Parasitology Biology Centre of the Academy of Sciences of the Czech Republic Branišovská 31 370 05 České Budějovice Czech Republic

Zobrazit více v PubMed

Folia Parasitol (Praha). 2013 May;60(2):177-80 PubMed

Infect Genet Evol. 2011 Aug;11(6):1276-86 PubMed

Int J Parasitol. 2010 Mar 1;40(3):333-43 PubMed

Can J Zool. 1977 Jan;55(1):64-73 PubMed

Int J Parasitol. 2013 Jan;43(1):57-72 PubMed

Infect Genet Evol. 2001 Dec;1(2):85-107 PubMed

Parasitology. 1966 Nov;56(4):693-706 PubMed

J Parasitol. 1957 Dec;43(6):613-27 PubMed

Int J Parasitol. 1995 Aug;25(8):897-906 PubMed

J Helminthol. 1973;47(4):409-14 PubMed

Parasitology. 2013 Apr;140(4):509-20 PubMed

Int J Parasitol. 2014 Sep;44(10):703-15 PubMed

Mol Biol Evol. 2013 Dec;30(12):2725-9 PubMed

Acta Parasitol. 2012 Sep;57(3):273-84 PubMed

J Helminthol. 1977 Jun;51(2):155-66 PubMed

Mol Ecol. 2010 Jul;19(13):2813-27 PubMed

Diversity of echinostomes (Digenea: Echinostomatidae) in their snail hosts at high latitudes

Unexpected diversity in northern Europe: trematodes from salmonid fishes in Iceland with two new species of Crepidostomum Braun, 1900

Exploring the diversity of Diplostomum (Digenea: Diplostomidae) in fishes from the River Danube using mitochondrial DNA barcodes

Trematode diversity in freshwater fishes of the Globe I: 'Old World'

Biodiversity of trematodes in their intermediate mollusc and fish hosts in the freshwater ecosystems of Europe

Integrative taxonomic approach to the cryptic diversity of Diplostomum spp. in lymnaeid snails from Europe with a focus on the 'Diplostomum mergi' species complex