UNLABELLED: Silicon nanocrystals (Si-ncs) are promising for biological studies due to their supposed low cytotoxicity, good biocompatibility and biodegradability in living organisms. However, the bioresearchers' focus on Si-ncs has lasted only for a few recent years, and detailed studies of the interaction of various types of Si-ncs with biological environment are still rare. Suitable size and solubility of the Si-ncs in water-based isotonic solutions are important towards bringing the nanocrystals inside the living cells. We have prepared colloidal solutions of luminescent porous silicon of different cluster sizes in methanol, water and phosphate-buffered saline (PBS). By combination of ultrasonic treatment with filtration, we have obtained two different silicon cluster sizes in methanol (120 and 525 nm) and three different cluster sizes (85, 210 and 1,500 nm) in PBS. Nanoclusters of heavily oxidized porous silicon are hydrophilic and well soluble in water and/or PBS. They can be further used for studies on the biocompatibility of these materials and may be potentially employed as luminescent markers in living cells in biological research. PACS: 78.67.Rb; 78.67.-n; 87.85.Qr; 87.85.Rs; 81.07.-b.

Department of Solid State Engineering Institute of Chemical Technology Prague 166 28 Czech Republic

Department of Thin Films and Nanostructures Institute of Physics Academy of Sciences of the Czech Republic v v i Cukrovarnická 10 162 53 Prague Czech Republic

Department of Thin Films and Nanostructures Institute of Physics Academy of Sciences of the Czech Republic v v i Cukrovarnická 10 162 53 Prague Czech Republic ; Department of Solid State Engineering Institute of Chemical Technology Prague 166 28 Czech Republic

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