The effect of exogenous 24-epibrassinolide on the ecdysteroid content in the leaves of Spinacia oleracea L
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25578736
DOI
10.1016/j.steroids.2014.12.024
PII: S0039-128X(15)00004-5
Knihovny.cz E-resources
- Keywords
- 20-Hydroxyecdysone, 24-Epibrassinolide, Ecdysteroids, Spinach,
- MeSH
- Brassinosteroids pharmacology MeSH
- Ecdysteroids metabolism MeSH
- Plant Leaves drug effects metabolism MeSH
- Molecular Conformation MeSH
- Spinacia oleracea chemistry drug effects MeSH
- Steroids, Heterocyclic pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- brassinolide MeSH Browser
- Brassinosteroids MeSH
- Ecdysteroids MeSH
- Steroids, Heterocyclic MeSH
The aim of this study was to show whether/how the application of exogenous 24-epibrassinolide can affect the content of ecdysteroids in spinach leaves. Brassinosteroids and ecdysteroids, structurally related phytosterols, show effect on a range of processes in plants. Brassinosteroids increase biomass yield in some species, photosynthesis and resistance to stress, and ecdysteroids show effect on proteins responsible for binding of CO2 or water cleavage. The mutual interaction of these sterols in plants is unclear. The UPLC-(+)ESI-MS/MS analyses of extracts of treated and untreated spinach (Spinacia oleracea L.) leaves show that the application of exogenous 24-epibrassinolide does influence the ecdysteroid content in plant tissues. The response differs for the major ecdysteroids and also differs from that for the minor ones and is dependent on the developmental stage of the leaves within the same plant or the 24-epibrassinolide concentration applied.
Dept of Genetics and Microbiology Faculty of Science Charles University Prague 12843 Czech Republic
Institute of Organic Chemistry and Biochemistry ASCR Prague 16610 Czech Republic
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