The effect of exogenous 24-epibrassinolide on the ecdysteroid content in the leaves of Spinacia oleracea L
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25578736
DOI
10.1016/j.steroids.2014.12.024
PII: S0039-128X(15)00004-5
Knihovny.cz E-zdroje
- Klíčová slova
- 20-Hydroxyecdysone, 24-Epibrassinolide, Ecdysteroids, Spinach,
- MeSH
- brassinosteroidy farmakologie MeSH
- ekdysteroidy metabolismus MeSH
- listy rostlin účinky léků metabolismus MeSH
- molekulární konformace MeSH
- Spinacia oleracea chemie účinky léků MeSH
- steroidy heterocyklické farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- brassinolide MeSH Prohlížeč
- brassinosteroidy MeSH
- ekdysteroidy MeSH
- steroidy heterocyklické MeSH
The aim of this study was to show whether/how the application of exogenous 24-epibrassinolide can affect the content of ecdysteroids in spinach leaves. Brassinosteroids and ecdysteroids, structurally related phytosterols, show effect on a range of processes in plants. Brassinosteroids increase biomass yield in some species, photosynthesis and resistance to stress, and ecdysteroids show effect on proteins responsible for binding of CO2 or water cleavage. The mutual interaction of these sterols in plants is unclear. The UPLC-(+)ESI-MS/MS analyses of extracts of treated and untreated spinach (Spinacia oleracea L.) leaves show that the application of exogenous 24-epibrassinolide does influence the ecdysteroid content in plant tissues. The response differs for the major ecdysteroids and also differs from that for the minor ones and is dependent on the developmental stage of the leaves within the same plant or the 24-epibrassinolide concentration applied.
Dept of Genetics and Microbiology Faculty of Science Charles University Prague 12843 Czech Republic
Institute of Organic Chemistry and Biochemistry ASCR Prague 16610 Czech Republic
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