Lanthanum rather than cadmium induces oxidative stress and metabolite changes in Hypericum perforatum
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25594936
DOI
10.1016/j.jhazmat.2014.12.060
PII: S0304-3894(14)01044-9
Knihovny.cz E-resources
- Keywords
- Antioxidants, Fluorescence microscopy, HPLC-MS/MS, Oxidative stress, Rare earth elements,
- MeSH
- Biomass MeSH
- Flavonoids metabolism MeSH
- Microscopy, Fluorescence MeSH
- Hydroxybenzoates metabolism MeSH
- Cadmium toxicity MeSH
- Plant Roots drug effects growth & development metabolism MeSH
- Lanthanum toxicity MeSH
- Environmental Pollutants toxicity MeSH
- Oxidative Stress drug effects MeSH
- Seedlings drug effects growth & development metabolism MeSH
- Hypericum drug effects growth & development metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Flavonoids MeSH
- Hydroxybenzoates MeSH
- Cadmium MeSH
- Lanthanum MeSH
- Environmental Pollutants MeSH
Physiology, oxidative stress and production of metabolites in Hypericum perforatum exposed to moderate Cd and/or La concentration (10 μM) were studied. La evoked increase in reactive oxygen species, malondialdehyde and proline but suppressed growth, tissue water content, glutathione, ascorbic acid and affected mineral nutrient contents more than Cd while the impact of Cd+La was not synergistic. Similar trend was observed at the level of superoxide dismutase gene expression. Shoot Cd amount increased in Cd+La while only root La increased in the same treatment. Extensive quantification of secondary metabolites revealed that La affected phenolic acids more pronouncedly than Cd in shoots and roots. Flavonols were suppressed by La that could contribute to the appearance of oxidative damage. Procyanidins increased in response to La in the shoots but decreased in the roots. Metabolic responses in Cd+La treatment resembled those of La treatment (almost identically in the roots). Phenylalanine ammonia-lyase activity was mainly suppressed by La. The presence of La also depleted amount of hypericin and expression of its putative gene (hyp-1) showed similar trend but accumulation of hyperforin increased under Cd or La excess. Clear differences in the stem and root anatomy in response to Cd or La were also found. Overall, H. perforatum is La-sensitive species and rather Cd ameliorated negative impact of La.
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