OBJECTIVE: A new interleukin-6 (IL-6)-dependent plasma cell leukemia cell line UHKT-944 was established from bone marrow cells derived from a 55-yr-old man with plasma cell leukemia. RESULTS: The cell line possesses phenotypic characteristics of plasma cells including the production of a monoclonal immunoglobulin IgA1-kappa. VH3-9 region of IgVH genes was rearranged and somatically hypermutated. The UHKT-944 cells were found to be negative for most of tested B-cell, T-cell, and myeloid markers. According to cytogenetic analysis, the cells were classified as near tetraploid with several numerical and structural abnormalities including the t(14;20) involving IgH locus. CONCLUSION: The established permanent plasma cell leukemia cell line is a suitable model for the study of cellular and molecular mechanisms of pathogenesis of this rare malignant disease.

1st Department of Internal Medicine Division of Hematology General Faculty Hospital Charles University Prague Czech Republic

Department of Biochemistry Faculty of Science Charles University Prague Czech Republic

Department of Cellular Biochemistry Institute of Hematology and Blood Transfusion Prague Czech Republic

Department of Cytogenetics IHBT Prague Czech Republic

Department of Experimental Virology IHBT Prague Czech Republic

Department of Immunology Faculty of Science Charles University Prague Czech Republic

Faculty of Arts Charles University Prague Czech Republic

Faculty of Science Charles University Prague Czech Republic

Leukemia PCR Diagnostics Laboratory IHBT Prague Czech Republic

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