Characterization of a new human plasma cell leukemia cell line UHKT-944
Language English Country Great Britain, England Media print-electronic
Document type Case Reports, Journal Article, Research Support, Non-U.S. Gov't
PubMed
25600340
DOI
10.1111/ejh.12505
Knihovny.cz E-resources
- Keywords
- human plasma cell line, interleukin-6 (IL-6), multiple myeloma, plasma cell, plasma cell leukemia,
- MeSH
- Biomarkers MeSH
- Cytogenetic Analysis MeSH
- Immunophenotyping MeSH
- Immunoglobulins genetics metabolism MeSH
- Middle Aged MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Leukemia, Plasma Cell diagnosis metabolism pathology MeSH
- Cell Proliferation MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Case Reports MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- Immunoglobulins MeSH
OBJECTIVE: A new interleukin-6 (IL-6)-dependent plasma cell leukemia cell line UHKT-944 was established from bone marrow cells derived from a 55-yr-old man with plasma cell leukemia. RESULTS: The cell line possesses phenotypic characteristics of plasma cells including the production of a monoclonal immunoglobulin IgA1-kappa. VH3-9 region of IgVH genes was rearranged and somatically hypermutated. The UHKT-944 cells were found to be negative for most of tested B-cell, T-cell, and myeloid markers. According to cytogenetic analysis, the cells were classified as near tetraploid with several numerical and structural abnormalities including the t(14;20) involving IgH locus. CONCLUSION: The established permanent plasma cell leukemia cell line is a suitable model for the study of cellular and molecular mechanisms of pathogenesis of this rare malignant disease.
Department of Biochemistry Faculty of Science Charles University Prague Czech Republic
Department of Cytogenetics IHBT Prague Czech Republic
Department of Experimental Virology IHBT Prague Czech Republic
Department of Immunology Faculty of Science Charles University Prague Czech Republic
Faculty of Arts Charles University Prague Czech Republic
Faculty of Science Charles University Prague Czech Republic
Leukemia PCR Diagnostics Laboratory IHBT Prague Czech Republic
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