The morphological, biological, and molecular characteristics of Cryptosporidium muris strain TS03 are described, and the species name Cryptosporidium proliferans n. sp. is proposed. Cryptosporidium proliferans obtained from a naturally infected East African mole rat (Tachyoryctes splendens) in Kenya was propagated under laboratory conditions in rodents (SCID mice and southern multimammate mice, Mastomys coucha) and used in experiments to examine oocyst morphology and transmission. DNA from the propagated C. proliferans isolate, and C. proliferans DNA isolated from the feces of an African buffalo (Syncerus caffer) in Central African Republic, a donkey (Equus africanus) in Algeria, and a domestic horse (Equus caballus) in the Czech Republic were used for phylogenetic analyses. Oocysts of C. proliferans are morphologically distinguishable from C. parvum and C. muris HZ206, measuring 6.8-8.8 (mean = 7.7 μm) × 4.8-6.2 μm (mean = 5.3) with a length to width ratio of 1.48 (n = 100). Experimental studies using an isolate originated from T. splendens have shown that the course of C. proliferans infection in rodent hosts differs from that of C. muris and C. andersoni. The prepatent period of 18-21 days post infection (DPI) for C. proliferans in southern multimammate mice (Mastomys coucha) was similar to that of C. andersoni and longer than the 6-8 DPI prepatent period for C. muris RN66 and HZ206 in the same host. Histopatologicaly, stomach glands of southern multimammate mice infected with C. proliferans were markedly dilated and filled with necrotic material, mucus, and numerous Cryptosporidium developmental stages. Epithelial cells of infected glands were atrophic, exhibited cuboidal or squamous metaplasia, and significantly proliferated into the lumen of the stomach, forming papillary structures. The epithelial height and stomach weight were six-fold greater than in non-infected controls. Phylogenetic analyses based on small subunit rRNA, Cryptosporidium oocyst wall protein, thrombospondin-related adhesive protein of Cryptosporidium-1, heat shock protein 70, actin, heat shock protein 90 (MS2), MS1, MS3, and M16 gene sequences revealed that C. proliferans is genetically distinct from C. muris and other previously described Cryptosporidium species.

CEITEC VFU Brno Czech Republic

Center for Food Safety Department of Food Science and Technology University of Georgia Griffin Georgia United States of America

Centers for Disease Control and Prevention Atlanta Georgia United States of America

Department of Pathology and Parasitology University of Veterinary and Pharmaceutical Sciences Brno Czech Republic

Faculty of Agriculture University of South Bohemia in České Budějovice České Budějovice Czech Republic

Faculty of Science University of South Bohemia in České Budějovice České Budějovice Czech Republic

Grammar School and High School of Economics Vimperk Czech Republic

Institute of Parasitology Biology Centre of the Czech Academy of Sciences České Budějovice Czech Republic

Veterinary and Microbiological Sciences Department North Dakota State University Fargo North Dakota United States of America

Zobrazit více v PubMed

O'Donoghue PJ. PubMed

Fayer R. General Biology In: Fayer R, Xiao L, editors.

Tyzzer EE. A sporozoan found in the peptic glands of the common mouse. Proc Soc Exp Biol Med. 1907;5:12.

Lindsay DS, Upton SJ, Owens DS, Morgan UM, Mead JR, Blagburn BL. PubMed

Upton SJ, McAllister CT, Freed PS, Barnard SM. PubMed

Morgan UM, Sargent KD, Deplazes P, Forbes DA, Spano F, Hertzberg H, et al. Molecular characterization of PubMed

Morgan UM, Xiao L, Fayer R, Graczyk TK, Lal AA, Deplazes P, et al. Phylogenetic analysis of PubMed

Traversa D, Iorio R, Otranto D, Modrý D, Šlapeta J. PubMed DOI

Ng J, Pavlásek I, Ryan U. Identification of novel PubMed DOI PMC

Xiao L, Ryan UM, Graczyk TK, Limor J, Li L, Kombert M, et al. Genetic diversity of PubMed PMC

Graczyk TK, Fayer R, Cranfield MR. PubMed

Crawshaw GJ, Mehren KG. Cryptosporidiosis in zoo and wild animals Erkrankungen der Zootiere Verhandlungsbericht des 29 Internationalen Symposiums Uber die Erkrankungen der Zootiere; Cardiff: Akademie-Verlag, Berlin; 1987. p. 353–62.

Jenkins MB, Liotta JL, Lucio-Forster A, Bowman DD. Concentrations, viability, and distribution of PubMed DOI PMC

Xiao L, Moore JE, Ukoh U, Gatei W, Lowery CJ, Murphy TM, et al. Prevalence and identity of PubMed DOI PMC

Kváč M, Hanzlíková D, Sak B, Květoňová D. Prevalence and age-related infection of PubMed DOI

Kváč M, Kestřánová M, Květoňová D, Kotková M, Ortega Y, McEvoy J, et al. PubMed DOI

Hikosaka K, Nakai Y. A novel genotype of PubMed DOI

Murakoshi F, Fukuda Y, Matsubara R, Kato Y, Sato R, Sasaki T, et al. Detection and genotyping of PubMed DOI

Feng Y, Yang W, Ryan U, Zhang L, Kváč M, Koudela B, et al. Development of a multilocus sequence tool for typing PubMed DOI PMC

Kváč M, Kodádková A, Sak B, Květoňová D, Jalovecká M, Rost M, et al. Activated CD8+ T cells contribute to clearance of gastric PubMed DOI

Kváč M, Sak B, Květoňová D, Secor WE. Infectivity of gastric and intestinal PubMed DOI

Kváč M, Sak B, Kvetoňová D, Ditrich O, Hofmannová L, Modrý D, et al. Infectivity, pathogenicity, and genetic characteristics of mammalian gastric PubMed DOI

Melicherová J, Ilgova J, Kváč M, Sak B, Koudela B, Valigurová A. Life cycle of PubMed DOI

Modrý D, Hofmannová L, Antalová Z, Sak B, Kváč M. Variability in susceptibility of voles (Arvicolinae) to experimental infection with PubMed DOI

Jalovecká M, Sak B, Kváč M, Květonová D, Kučerová Z, Salát J. Activation of protective cell-mediated immune response in gastric mucosa during PubMed DOI

Chalmers RM, Sturdee AP, Bull SA, Miller A, Wright SE. The prevalence of PubMed

Aydin Y, Ozkul IA. Infectivity of PubMed

Hůrková L, Hajdušek O, Modrý D. Natural infection of PubMed DOI

Kváč M, Ondráčková Z, Květoňová D, McEvoy J, Vítovec J, Rost M, et al. The Lesser Egyptian Gerbil ( PubMed DOI

Koudela B, Modrý D, Vítovec J. Infectivity of PubMed

Ryan U, Xiao L, Read C, Zhou L, Lal AA, Pavlásek I. Identification of novel PubMed PMC

Neumayerová H, Koudela B. Effects of low and high temperatures on infectivity of PubMed DOI

Lv C, Zhang L, Wang R, Jian F, Zhang S, Ning C, et al. Cryptosporidium spp. in wild, laboratory, and pet rodents in china: prevalence and molecular characterization. Appl Environ Microbiol. 2009;75(24):7692–9. 10.1128/AEM.01386-09 PubMed DOI PMC

Rhee JK, So WS, Kim HC. Age-dependent resistance to PubMed PMC

Satoh M, Hikosaka K, Sasaki T, Suyama Y, Yanai T, Ohta M, et al. Characteristics of a novel type of bovine PubMed PMC

Pavlásek I, Lávička M. [The first finding of a spontaneous gastric cryptosporidiosis infection in hamsters ( PubMed

Iseki M, Maekawa T, Moriya K, Uni S, Takada S. Infectivity of PubMed

Feng Y, Alderisio KA, Yang W, Blancero LA, Kuhne WG, Nadareski CA, et al. PubMed DOI PMC

Warren KS, Swan RA, Morgan-Ryan UM, Friend JA, Elliot A. PubMed

Esteban E, Anderson BC. PubMed DOI

Trout JM, Santín M, Fayer R. PubMed

Dixon BR, Parrington LJ, Parenteau M, Leclair D, Santín M, Fayer R. Giardia duodenalis and PubMed DOI

Qin SY, Zhang XX, Zhao GH, Zhou DH, Yin MY, Zhao Q, et al. First report of PubMed DOI PMC

Rhee JK, Kim HC, Eun GS. Infection kinetics and developmental biology of PubMed PMC

Ryan UM, Bath C, Robertson I, Read C, Elliot A, McInnes L, et al. Sheep may not be an important zoonotic reservoir for PubMed DOI PMC

Anderson BC. Experimental infection in mice of PubMed

Pospischil A, Stiglmair-Herb MT, von Hegel G, Wiesner H. Abomasal cryptosporidiosis in mountain gazelles. Vet Rec. 1987;121(16):379–80. . PubMed

Kodádková A, Kváč M, Ditrich O, Sak B, Xiao L. PubMed DOI

Deng MQ, Cliver DO. Improved immunofluorescence assay for detection of PubMed

Valigurová A, Hofmannová L, Koudela B, Vávra J. An ultrastructural comparison of the attachment sites between PubMed

Siefker C, Rickard LG, Pharr GT, Simmons JS, O'Hara TM. Molecular characterization of PubMed DOI

Sak B, Petrželková KJ, Květoňová D, Mynařová A, Shutt KA, Pomajbíková K, et al. Long-term monitoring of microsporidia, PubMed DOI PMC

Laatamna AE, Wagnerová P, Sak B, Květoňová D, Xiao L, Rost M, et al. Microsporidia and PubMed DOI

Wagnerová P, Sak B, McEvoy J, Rost M, Perec Matysiak A, Ježková J, et al. Genetic diversity of PubMed DOI

Katsumata T, Hosea D, Ranuh IG, Uga S, Yanagi T, Kohno S. Short report: possible PubMed

Guyot K, Follet-Dumoulin A, Lelievre E, Sarfati C, Rabodonirina M, Nevez G, et al. Molecular characterization of PubMed PMC

Dubey JP, Markovits JE, Killary KA. PubMed

Karim MR, Zhang S, Jian F, Li J, Zhou C, Zhang L, et al. Multilocus typing of PubMed DOI

Qi M, Huang L, Wang R, Xiao L, Xu L, Li J, et al. Natural infection of PubMed DOI

Tilley M, Upton SJ, Chrisp CE. A comparative study on the biology of PubMed

Pedraza-Diaz S, Ortega-Mora LM, Carrion BA, Navarro V, Gomez-Bautista M. Molecular characterisation of PubMed DOI

Rhee JK, Yook SY, Park BK. Oocyst production and immunogenicity of PubMed

Matsue T, Fujino T, Kajima J, Tsuji M. Infectivity and oocyst excretion patterns of PubMed

Kváč M, Ondráčková Z, Květoňová D, Sak B, Vítovec J. Infectivity and pathogenicity of PubMed DOI

Miláček P, Vítovec J. Differential staining of cryptosporidia by aniline-carbol-methyl violet and tartrazine in smears from feces and scrapings of intestinal mucosa. Folia Parasitol. 1985;32(1):50 Epub 1985/01/01. . PubMed

Arrowood MJ, Sterling CR. Isolation of PubMed

Kilani RT, Sekla L. Purification of PubMed

Sauch JF, Flanigan D, Galvin ML, Berman D, Jakubowski W. Propidium iodide as an indicator of PubMed PMC

Casemore DP. ACP Broadsheet 128: June 1991. Laboratory methods for diagnosing cryptosporidiosis. J Clin Pathol. 1991;44(6):445–51. PubMed PMC

Jiang J, Alderisio KA, Xiao L. Distribution of PubMed DOI PMC

Xiao L, Morgan UM, Limor J, Escalante A, Arrowood M, Shulaw W, et al. Genetic diversity within PubMed PMC

Sulaiman IM, Lal AA, Xiao L. Molecular phylogeny and evolutionary relationships of PubMed

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28(10):2731–9. 10.1093/molbev/msr121 PubMed DOI PMC

Guindon S, Gascuel O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003;52(5):696–704. . PubMed

Kváč M, Vítovec J. Prevalence and pathogenicity of PubMed

Tyzzer EE.

Fayer R, Speer CA, Dubey JP. The general biology of

Xiao L, Escalante L, Yang C, Sulaiman I, Escalante AA, Montali RJ, et al. Phylogenetic analysis of PubMed PMC

Ren X, Zhao J, Zhang L, Ning C, Jian F, Wang R, et al. PubMed DOI

Fayer R, Trout JM, Xiao L, Morgan UM, Lai AA, Dubey JP. PubMed DOI

Morgan-Ryan UM, Fall A, Ward LA, Hijjawi N, Sulaiman I, Fayer R, et al. PubMed

Ng-Hublin JS, Singleton GR, Ryan U. Molecular characterization of PubMed DOI

Sréter T, Egyed Z, Szell Z, Kovacs G, Nikolausz M, Marialigeti K, et al. Morphologic, host specificity, and genetic characterization of a European PubMed DOI

Fayer R, Santín M. PubMed DOI

Vítovec J, Hamadejová K, Landová L, Kváč M, Květoňová D, Sak B. Prevalence and pathogenicity of PubMed DOI

Kváč M, Hofmannová L, Hlásková L, Květoňová D, Vítovec J, McEvoy J, et al. PubMed DOI

Robinson G, Wright S, Elwin K, Hadfield SJ, Katzer F, Bartley PM, et al. Re-description of PubMed DOI

Kváč M, McEvoy J, Loudová M, Stenger B, Sak B, Květoňová D, et al. Coevolution of PubMed DOI PMC

Nakai Y, Hikosaka K, Sato M, Sasaki T, Kaneta Y, Okazaki N. Detection of PubMed

Taylor MA, Marshall RN, Green JA, Catchpole J. The pathogenesis of experimental infections of PubMed

McDonald V, Deer R, Uni S, Iseki M, Bancroft GJ. Immune responses to PubMed PMC

Anderson BC. Abomasal cryptosporidiosis in cattle. Vet Pathol. 1987;24(3):235–8. . PubMed

Tyzzer EE. An extracellular coccidium, PubMed PMC

Ozkul IA, Aydin Y. Natural PubMed

Anderson BC. Cryptosporidiosis in bovine and human health. J Dairy Sci. 1998;81(11):3036–41. PubMed

Koyama Y, Satoh M, Maekawa K, Hikosaka K, Nakai Y. Isolation of PubMed DOI

Cryptosporidium geckonae n. sp. (Apicomplexa: Cryptosporidiidae) in geckos

Waterborne protozoan and microsporidian parasites in Eurasian beavers (Castor fiber)

Cryptosporidium mortiferum n. sp. (Apicomplexa: Cryptosporidiidae), the species causing lethal cryptosporidiosis in Eurasian red squirrels (Sciurus vulgaris)

Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus)

Cryptosporidium ratti n. sp. (Apicomplexa: Cryptosporidiidae) and genetic diversity of Cryptosporidium spp. in brown rats (Rattus norvegicus) in the Czech Republic

NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome

Cryptosporidium infecting wild cricetid rodents from the subfamilies Arvicolinae and Neotominae

Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016