Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland

. 2015 Apr ; 114 (4) : 1619-24. [epub] 20150227

Jazyk angličtina Země Německo Médium print-electronic

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid25722018

Faecal samples were collected from 352 horses on 23 farms operating under six different management systems in the Czech Republic and Poland during 2011 and 2012. Farms were selected without previous knowledge of parasitological status. All faecal samples were screened for Cryptosporidium spp. presence using microscopy, following aniline-carbol-methyl violet staining and PCR analysis of the small-subunit (SSU) rRNA and the 60-kDa glycoprotein (gp60) genes. Cryptosporidium muris-positive samples were additionally genotyped at four minisatellite markers: MS1 (encoding a hypothetical protein), MS2 (encoding a 90-kDa heat shock protein), MS3 (encoding a hypothetical protein) and MS16 (encoding a leucine-rich repeat family protein). Cryptosporidium spp. was detected by PCR in 12/352 (3.4%) samples from 4 out of 13 farms. None of the samples tested by microscopy was positive. There was no relationship between Cryptosporidium prevalence and age, sex, diarrhoea or management system; however, Cryptosporidium was found only on farms where horses were kept on pasture during the day and in a stable overnight. Sequence analyses of SSU and gp60 genes revealed the presence of C. muris RN66 (n = 9), Cryptosporidium parvum IIaA15G2R1 (n = 1), Cryptosporidium tyzzeri IXbA22R9 (n = 1), and Cryptosporidium horse genotype VIaA15G4 (n = 1). The C. muris subtypes were identified as MS1-M1, MS2-M4, novel MS2-M7 and MS16-M1 by multilocus sequence of three minisatellite loci. The MS3 locus was not amplified from any isolate. This is the first report of C. tyzzeri and C. muris subtypes from horses.

Zobrazit více v PubMed

J Clin Microbiol. 2008 Jul;46(7):2396-8 PubMed

J Clin Microbiol. 2005 Jun;43(6):2805-9 PubMed

Int J Parasitol. 2008 Jun;38(7):809-17 PubMed

Equine Vet J. 1994 Jan;26(1):14-7 PubMed

J Clin Microbiol. 2011 Jan;49(1):34-41 PubMed

Vet Rec. 2003 Nov 15;153(20):628-31 PubMed

Mol Biol Evol. 2011 Oct;28(10):2731-9 PubMed

Vet Parasitol. 2011 Dec 15;182(2-4):333-6 PubMed

PLoS One. 2012;7(8):e43782 PubMed

Vet Parasitol. 2006 Oct 10;141(1-2):48-59 PubMed

J Clin Microbiol. 2003 Jun;41(6):2744-7 PubMed

Emerg Infect Dis. 2002 Jun;8(6):631-3 PubMed

Vet Parasitol. 1997 Mar;68(4):375-81 PubMed

Microbes Infect. 2004 Jul;6(8):773-85 PubMed

Vet Pathol. 1978 Jan;15(1):12-7 PubMed

Zoonoses Public Health. 2010 Dec;57(7-8):510-7 PubMed

J Clin Microbiol. 2014 Jan;52(1):347-9 PubMed

Parasitol Res. 2004 Jul;93(4):274-8 PubMed

Appl Environ Microbiol. 2005 Aug;71(8):4446-54 PubMed

Vet Parasitol. 2010 Nov 24;174(1-2):139-44 PubMed

Can Vet J. 1985 Apr;26(4):132-4 PubMed

Parasitol Res. 2006 Sep;99(4):346-52 PubMed

Folia Parasitol (Praha). 1985;32(1):50 PubMed

Vet J. 2013 Nov;198(2):531-3 PubMed

Vet Parasitol. 2009 Mar 23;160(3-4):327-33 PubMed

Vet Parasitol. 1999 Jun 1;83(1):1-13 PubMed

Vet Parasitol. 2013 Oct 18;197(1-2):350-3 PubMed

Aust Vet J. 2000 Jan;78(1):27-8 PubMed

N Z Vet J. 2009 Oct;57(5):284-9 PubMed

J Clin Microbiol. 2009 Sep;47(9):3017-20 PubMed

Int J Parasitol. 2013 Sep;43(10):805-17 PubMed

J Am Vet Med Assoc. 1998 Nov 1;213(9):1296-302 PubMed

Vet Parasitol. 2003 Oct 8;116(2):97-113 PubMed

Appl Environ Microbiol. 2003 Jul;69(7):4302-7 PubMed

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...