Plasma levels of 7-hydroxylated dehydroepiandrosterone (DHEA) metabolites and selected amino-thiols as discriminatory tools of Alzheimer's disease and vascular dementia
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15202788
DOI
10.1515/cclm.2004.088
Knihovny.cz E-zdroje
- MeSH
- 17-alfa-hydroxypregnenolon krev MeSH
- Alzheimerova nemoc krev diagnóza MeSH
- biologické markery krev MeSH
- cystein analogy a deriváty krev MeSH
- dehydroepiandrosteron analogy a deriváty krev MeSH
- dehydroepiandrosteronsulfát krev MeSH
- dipeptidy krev MeSH
- glutathion krev MeSH
- homocystein krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- logistické modely MeSH
- neparametrická statistika MeSH
- prediktivní hodnota testů MeSH
- pregnenolon krev MeSH
- radioimunoanalýza MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- senzitivita a specificita MeSH
- sexuální faktory MeSH
- sulfhydrylové sloučeniny krev MeSH
- vaskulární demence krev diagnóza MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 17-alfa-hydroxypregnenolon MeSH
- 7-hydroxydehydroepiandrosterone MeSH Prohlížeč
- biologické markery MeSH
- cystein MeSH
- cysteinylglycine MeSH Prohlížeč
- dehydroepiandrosteron MeSH
- dehydroepiandrosteronsulfát MeSH
- dipeptidy MeSH
- glutathion MeSH
- homocystein MeSH
- pregnenolon MeSH
- pregnenolone sulfate MeSH Prohlížeč
- sulfhydrylové sloučeniny MeSH
OBJECTIVE: The early differential diagnosis of Alzheimer's disease (AD) remains still problematic. We developed a laboratory test enabling us to distinguish patients with AD from those with vascular dementia (VD) and healthy subjects. METHODS: The AD group consisted of 22 women and 18 men. The VD group consisted of 16 women and 8 men. Age-matched controls consisted of 12 women and 9 men. Plasma pregnenolone sulfate (PregS), dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) were determined by radioimmunoassay. 17-Hydroxypregnenolone (17Preg) and 7-hydroxylated metabolites of DHEA (7alphaDHEA, 7betaDHEA) were determined by radioimmunoassay after separation by high performance liquid chromatography (HPLC). Homocysteine (Hcy), cysteine (Cys), cysteinylglycine (Cysgly) and glutathione (GSH) were measured by HPLC. RESULTS: The ANOVA results of significant between-group differences were as follows: The PregS and the 17-Preg and DHEAS levels were independent from the diagnosis. The 7alphaDHEA levels significantly depended on the sex (p < 0.05) and diagnosis (p < 0.01). Amino-thiols were influenced by the diagnosis (p < 0.01, p = 0.0541, p < 0.01 and p = 0.0536 for Cys, Hcy, Cysgly and GSH, respectively). Using a stepwise backward regression analysis, the following parameters were obtained: X = 11.5 + 4.03 x sex +1.09 x Hcy + 0.190 x PregS - 4.76 x DHEAS + 3.00 x DHEA - 34.3 x 77alphaDHEA - 0.885 x Cysgly from which P-value as a discriminator was calculated according to the formula: P = 1/(1 + e(-x)). Then, for P > 0.5, a subject was considered as AD-positive (with 89% correct prediction). DISCUSSION: The opportunity of early differential diagnosis of AD should help physicians to use suitable treatment for retardation of pathological processes.
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