Cryptosporidium ratti n. sp. (Apicomplexa: Cryptosporidiidae) and genetic diversity of Cryptosporidium spp. in brown rats (Rattus norvegicus) in the Czech Republic
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
32981543
PubMed Central
PMC11010154
DOI
10.1017/s0031182020001833
PII: S0031182020001833
Knihovny.cz E-zdroje
- Klíčová slova
- Cryptosporidium ratti, infectivity, morphometric analysis, phylogeny, prevalence,
- MeSH
- aktiny genetika MeSH
- Cryptosporidium * klasifikace genetika izolace a purifikace MeSH
- divoká zvířata parazitologie MeSH
- feces parazitologie MeSH
- fylogeneze MeSH
- genetická variace MeSH
- klasifikace MeSH
- krysa rodu Rattus parazitologie MeSH
- myši MeSH
- prevalence MeSH
- proteiny tepelného šoku HSP70 genetika MeSH
- protozoální DNA MeSH
- ribozomální DNA genetika MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus parazitologie MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aktiny MeSH
- proteiny tepelného šoku HSP70 MeSH
- protozoální DNA MeSH
- ribozomální DNA MeSH
The diversity and biology of Cryptosporidium that is specific for rats (Rattus spp.) are not well studied. We examined the occurrence and genetic diversity of Cryptosporidium spp. in wild brown rats (Rattus norvegicus) by microscopy and polymerase chain reaction (PCR)/sequencing targeting the small subunit rDNA (SSU), actin and HSP70 genes. Out of 343 faecal samples tested, none were positive by microscopy and 55 were positive by PCR. Sequence analysis of SSU gene revealed the presence of Cryptosporidium muris (n = 4), C. andersoni (n = 3), C. ryanae (n = 1), C. occultus (n = 3), Cryptosporidium rat genotype I (n = 23), Cryptosporidium rat genotype IV (n = 16) and novel Cryptosporidium rat genotype V (n = 5). Spherical oocysts of Cryptosporidium rat genotype I obtained from naturally-infected rats, measuring 4.4-5.4 μm × 4.3-5.1 μm, were infectious to the laboratory rats, but not to the BALB/c mice (Mus musculus) nor Mongolian gerbils (Meriones unguiculatus). The prepatent period was 3 days post infection and the patent period was longer than 30 days. Naturally- and experimentally-infected rats showed no clinical signs of disease. Percentage of nucleotide similarities at the SSU, actin, HSP70 loci between C. ratti n. sp. and the rat derived C. occultus and Cryptosporidium rat genotype II, III, IV, and V ranged from 91.0 to 98.1%. These genetic variations were similar or greater than that observed between closely related species, i.e. C. parvum and C. erinacei (93.2-99.5%). Our morphological, genetic and biological data support the establishment of Cryptosporidium rat genotype I as a new species, Cryptosporidium ratti n. sp.
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