Cryptosporidium spp., common parasites of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutrias (Myocastor coypus). A total of 150 faecal samples of feral nutria were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci. Molecular analyses revealed the presence of C. parvum (n = 1), C. ubiquitum subtype family XIId (n = 5) and Cryptosporidium myocastoris n. sp. XXIIa (n = 2), and XXIIb (n = 3). Only nutrias positive for C. myocastoris shed microscopically detectable oocysts, which measured 4.8-5.2 × 4.7-5.0 µm, and oocysts were infectious for experimentally infected nutrias with a prepatent period of 5-6 days, although not for mice, gerbils, or chickens. The infection was localised in jejunum and ileum without observable macroscopic changes. The microvilli adjacent to attached stages responded by elongating. Clinical signs were not observed in naturally or experimentally infected nutrias. Phylogenetic analyses at SSU, actin, and HSP70 loci demonstrated that C. myocastoris n. sp. is distinct from other valid Cryptosporidium species.

Faculty of Agriculture University of South Bohemia in České Budějovice Studentská 1668 37005 České Budějovice Czech Republic

Faculty of Forestry Technical University in Zvolen 960 01 Zvolen Slovakia

Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642 China

Institute of Parasitology Biology Centre of the Czech Academy of Sciences Branišovská 31 37005 České Budějovice Czech Republic

Key Laboratory of Zoonosis of Ministry of Agriculture College of Veterinary Medicine South China Agricultural University Guangzhou 510642 China

Microbiological Sciences Department North Dakota State University 1523 Centennial Blvd Van Es Hall Fargo ND 58102 USA

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Fayer R. Taxonomy and species delimitation in Cryptosporidium. Exp. Parasitol. 2010;124:90–97. doi: 10.1016/j.exppara.2009.03.005. PubMed DOI

Ryan U., Xiao L. Taxonomy and Molecular Taxonomy. In: Cacciò S.M., Widmer G., editors. Cryptosporidium: Parasite and Disease. 1st ed. Springer; Berlin/Heidelberg, Germany: 2014. pp. 3–42.

Nakamura A.A., Meireles M.V. Cryptosporidium infections in birds—A review. Rev. Bras. Parasitol. Vet. 2015;24:253–267. doi: 10.1590/S1984-29612015063. PubMed DOI

Zintl A., Neville D., Maguire D., Fanning S., Mulcahy G., Smith H.V., De Waal T. Prevalence of Cryptosporidium species in intensively farmed pigs in Ireland. Parasitology. 2007;134:1575–1582. doi: 10.1017/S0031182007002983. PubMed DOI

Čondlová S., Horčičková M., Havrdová N., Sak B., Hlásková L., Perec-Matysiak A., Kicia M., McEvoy J., Kváč M. Diversity of Cryptosporidium spp. in Apodemus spp. in Europe. Eur. J. Protistol. 2019;69:1–13. doi: 10.1016/j.ejop.2019.02.005. PubMed DOI PMC

Horčičková M., Čondlová S., Holubová N., Sak B., Květoňová D., Hlásková L., Konečný R., Sedláček F., Clark M., Giddings C., et al. Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae) Parasitology. 2019;146:220–233. doi: 10.1017/S0031182018001142. PubMed DOI PMC

Stenger B.L.S., Horčičková M., Clark M.E., Kváč M., Čondlová S., Khan E., Widmer G., Xiao L., Giddings C.W., Pennil C., et al. Cryptosporidium infecting wild cricetid rodents from the subfamilies Arvicolinae and Neotominae. Parasitology. 2017;145:326–334. doi: 10.1017/S0031182017001524. PubMed DOI PMC

Wei Z., Liu Q., Zhao W., Jiang X., Zhang Y., Zhao A., Jing B., Lu G., Qi M. Prevalence and diversity of Cryptosporidium spp. in bamboo rats (Rhizomys sinensis) in South Central China. Int. J. Parasitol. Parasites Wildl. 2019;9:312–316. doi: 10.1016/j.ijppaw.2019.06.010. PubMed DOI PMC

Ryan U., Paparini A., Tong K.S., Yang R.C., Gibson-Kueh S., O’Hara A., Lymbery A., Xiao L.H. Cryptosporidium huwi n. sp (Apicomplexa: Eimeriidae) from the guppy (Poecilia reticulata) Exp. Parasitol. 2015;150:31–35. doi: 10.1016/j.exppara.2015.01.009. PubMed DOI

Zahedi A., Durmic Z., Gofton A.W., Kueh S., Austen J., Lawson M., Callahan L., Jardine J., Ryan U. Cryptosporidium homai n. sp. (Apicomplexa: Cryptosporidiiae) from the guinea pig (Cavia porcellus) Vet. Parasitol. 2017;245:92–101. doi: 10.1016/j.vetpar.2017.08.014. PubMed DOI

Holubová N., Zikmundová V., Limpouchová Z., Sak B., Konečný R., Hlásková L., Rajský D., Kopacz Z., McEvoy J., Kváč M. Cryptosporidium proventriculi sp. n. (Apicomplexa: Cryptosporidiidae) in Psittaciformes birds. Eur. J. Protistol. 2019;69:70–87. doi: 10.1016/j.ejop.2019.03.001. PubMed DOI

Ježková J., Prediger J., Holubová N., Sak B., Konečný R., Feng Y., Xiao L., Rost M., McEvoy J., Kváč M. Cryptosporidium ratti n. sp. (Apicomplexa: Cryptosporidiidae) and genetic diversity of Cryptosporidium spp. in brown rats (Rattus norvegicus) in the Czech Republic. Parasitology. 2021;148:84–97. doi: 10.1017/S0031182020001833. PubMed DOI PMC

Zahedi A., Bolland S.J., Oskam C.L., Ryan U. Cryptosporidium abrahamseni n. sp. (Apicomplexa: Cryptosporidiiae) from red-eye tetra (Moenkhausia sanctaefilomenae) Exp. Parasitol. 2021;223:108089. doi: 10.1016/j.exppara.2021.108089. PubMed DOI

Fayer R., Xiao L. Cryptosporidium and Cryptosporidiosis. CRC Press; New York, NY, USA: 2007. p. 560.

Martino P.E., Radman N., Parrado E., Bautista E., Cisterna C., Silvestrini M.P., Corba S. Note on the occurrence of parasites of the wild nutria (Myocastor coypus, Molina, 1782) Helminthologia. 2012;49:164–168. doi: 10.2478/s11687-012-0033-y. DOI

Tsiamis K., Gervasini E., Deriu I., D’Amico F., Nunes A.L., Addamo A.M., Cardoso A.C. In: Baseline Distribution of Invasive Alien Species of Union Concern. Commision E., editor. JRC Science for Poplicy Report; Luxembourg: 2017. p. 96. DOI

Martino P.E., Radman N.E., Gamboa M.I., Samartino L.E., Parrado E.J. Ectoparasites from some Myocastor coypus (Molina, 1782) populations (Coypus or Nutria) in Argentina. Rev. Bras. Parasitol. Vet. 2018;27:254–257. doi: 10.1590/s1984-296120180026. PubMed DOI

Kellnerová K., Holubová N., Jandová A., Vejčík A., McEvoy J., Sak B., Kváč M. First description of Cryptosporidium ubiquitum XIIa subtype family in farmed fur animals. Eur. J. Protistol. 2017;59:108–113. doi: 10.1016/j.ejop.2017.03.007. PubMed DOI

Babero B.B., Lee J.W. Studies on the helminths of nutria, Myocastor coypus (Molina), in Louisiana with check-list of other worm parasites from this host. J. Parasitol. 1961;47:378–390. doi: 10.2307/3275359. PubMed DOI

Pavlásek I., Kozakiewicz B. Coypus (Myocastor coypus) as a new host of Cryptosporidium parvum (Apicomplexa: Cryptosporidiidae) Folia Parasitol. (Praha) 1991;38:90. PubMed

Zanzani S.A., Di Cerbo A., Gazzonis A.L., Epis S., Invernizzi A., Tagliabue S., Manfredi M.T. Parasitic and Bacterial Infections of Myocastor coypus in a Metropolitan Area of Northwestern Italy. J. Wildl. Dis. 2016;52:126–130. doi: 10.7589/2015-01-010. PubMed DOI

Miláček P., Vítovec J. Differential staining of cryptosporidia by aniline-carbol-methyl violet and tartrazine in smears from feces and scrapings of intestinal mucosa. Folia Parasitol. 1985;32:50. PubMed

Kváč M., Ondráčková Z., Květoňová D., Sak B., Vítovec J. Infectivity and pathogenicity of Cryptosporidium andersoni to a novel host, southern multimammate mouse (Mastomys coucha) Vet. Parasitol. 2007;143:229–233. doi: 10.1016/j.vetpar.2006.08.031. PubMed DOI

Jiang J., Alderisio K.A., Xiao L. Distribution of Cryptosporidium genotypes in storm event water samples from three watersheds in New York. Appl. Environ. Microbiol. 2005;71:4446–4454. doi: 10.1128/AEM.71.8.4446-4454.2005. PubMed DOI PMC

Xiao L., Morgan U.M., Limor J., Escalante A., Arrowood M., Shulaw W., Thompson R.C., Fayer R., Lal A.A. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl. Environ. Microbiol. 1999;65:3386–3391. doi: 10.1128/AEM.65.8.3386-3391.1999. PubMed DOI PMC

Sulaiman I.M., Lal A.A., Xiao L.H. Molecular phylogeny and evolutionary relationships of Cryptosporidium parasites at the actin locus. J. Parasitol. 2002;88:388–394. doi: 10.1645/0022-3395(2002)088[0388:MPAERO]2.0.CO;2. PubMed DOI

Sulaiman I.M., Morgan U.M., Thompson R.C., Lal A.A., Xiao L. Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene. Appl. Environ. Microbiol. 2000;66:2385–2391. doi: 10.1128/AEM.66.6.2385-2391.2000. PubMed DOI PMC

Alves M., Xiao L.H., Sulaiman I., Lal A.A., Matos O., Antunes F. Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J. Clin. Microbiol. 2003;41:2744–2747. doi: 10.1128/JCM.41.6.2744-2747.2003. PubMed DOI PMC

Li N., Xiao L., Alderisio K., Elwin K., Cebelinski E., Chalmers R., Santin M., Fayer R., Kváč M., Ryan U., et al. Subtyping Cryptosporidium ubiquitum, a zoonotic pathogen emerging in humans. Emerg. Infect. Dis. 2014;20:217–224. doi: 10.3201/eid2002.121797. PubMed DOI PMC

Hall T.A. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT Nucl. Acids. Symp. Ser. 1999;41:95–98.

Kumar S., Stecher G., Li M., Knyaz C., Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol. Biol. Evol. 2018;35:1547–1549. doi: 10.1093/molbev/msy096. PubMed DOI PMC

Kearse M., Moir R., Wilson A., Stones-Havas S., Cheung M., Sturrock S., Buxton S., Cooper A., Markowitz S., Duran C., et al. Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 2012;28:1647–1649. doi: 10.1093/bioinformatics/bts199. PubMed DOI PMC

Arrowood M.J., Donaldson K. Improved purification methods for calf-derived Cryptosporidium parvum oocysts using discontinuous sucrose and cesium chloride gradients. J. Eukaryot. Microbiol. 1996;43:89S. doi: 10.1111/j.1550-7408.1996.tb05015.x. PubMed DOI

Arrowood M.J., Sterling C.R. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J. Parasitol. 1987;73:314–319. doi: 10.2307/3282084. PubMed DOI

Henriksen S.A., Pohlenz J.F. Staining of cryptosporidia by a modified Ziehl-Neelsen technique. Acta Vet. Scand. 1981;22:594–596. doi: 10.1186/BF03548684. PubMed DOI PMC

Kváč M., Vítovec J. Prevalence and pathogenicity of Cryptosporidium andersoni in one herd of beef cattle. J. Vet. Med. B. 2003;50:451–457. doi: 10.1046/j.0931-1793.2003.00701.x. PubMed DOI

Nordhausen K., Sirkia S., Oja H., Tyler D. CSNP: Tools for Multivariate Nonparametrics. R package version 1.1-1. [(accessed on 20 February 2021)]; Available online: https://CRAN.R-project.org/package=ICSNP.

R Core Team . R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing; Vienna, Austria: 2019.

Kváč M., Horčičková M., Holubová N., Květoňová D., Hlásková L., McEvoy J., Rajský D., Sak B. Cryptosporidium ubiquitum and Cryptosporidium coypu genotype in wild coypu (Myocastor coypus); Proceedings of the 14th Interantional Congress of Parasitology; Daegu, Korea. 19–24 August 2010.

ICZN International Commission on Zoological Nomenclature: Amendment of articles 8, 9, 10, 21 and 78 of the international code of zoologicalnomenclature to expand and refine methods of publication. Bull. Zool. Nomencl. 2012;69:161–169. doi: 10.21805/bzn.v69i3.a8.161. PubMed DOI

Meireles M.V., Soares R.M., Bonello F., Gennari S.M. Natural infection with zoonotic subtype of Cryptosporidium parvum in Capybara (Hydrochoerus hydrochaeris) from Brazil. Vet. Parasitol. 2007;147:166–170. doi: 10.1016/j.vetpar.2007.03.034. PubMed DOI

Zhou L., Fayer R., Trout J.M., Ryan U.M., Schaefer F.W., 3rd, Xiao L. Genotypes of Cryptosporidium species infecting fur-bearing mammals differ from those of species infecting humans. Appl. Environ. Microbiol. 2004;70:7574–7577. doi: 10.1128/AEM.70.12.7574-7577.2004. PubMed DOI PMC

Paziewska A., Bednarska M., Nieweglowski H., Karbowiak G., Bajer A. Distribution of Cryptosporidium and Giardia spp. in selected species of protected and game mammals from north-eastern Poland. Ann. Agric. Environ. Med. 2007;14:265–270. PubMed

Nasser A.M. Removal of Cryptosporidium by wastewater treatment processes: A review. J. Water Health. 2016;14:1–13. doi: 10.2166/wh.2015.131. PubMed DOI

Hamilton K.A., Waso M., Reyneke B., Saeidi N., Levine A., Lalancette C., Besner M.C., Khan W., Ahmed W. Cryptosporidium and Giardia in Wastewater and Surface Water Environments. J. Environ. Qual. 2018;47:1006–1023. doi: 10.2134/jeq2018.04.0132. PubMed DOI

Smith H.V., Nichols R.A.B. Cryptosporidium: Detection in water and food. Exp. Parasitol. 2010;124:61–79. doi: 10.1016/j.exppara.2009.05.014. PubMed DOI

Feng Y. Cryptosporidium in wild placental mammals. Exp. Parasitol. 2010;124:128–137. doi: 10.1016/j.exppara.2008.11.005. PubMed DOI

Perz J.F., Le Blancq S.M. Cryptosporidium parvum infection involving novel genotypes in wildlife from lower New York State. Appl. Environ. Microbiol. 2001;67:1154–1162. doi: 10.1128/AEM.67.3.1154-1162.2001. PubMed DOI PMC

Hattori K., Donomoto T., Manchanayake T., Shibahara T., Sasai K., Matsubayashi M. First surveillance and molecular identification of the Cryptosporidium skunk genotype and Cryptosporidium parvum in wild raccoons (Procyon lotor) in Osaka, Japan. Parasitol. Res. 2018;117:3669–3674. doi: 10.1007/s00436-018-6089-y. PubMed DOI

Huang J.Y., Zhang Z.J., Zhang Y.Q., Yang Y., Zhao J.F., Wang R.J., Jian F.C., Ning C.S., Zhang W.Y., Zhang L.X. Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in deer in Henan and Jilin, China. Parasit. Vectors. 2018;11:239. doi: 10.1186/s13071-018-2813-9. PubMed DOI PMC

Kváč M., McEvoy J., Stenger B., Clark M. Cryptosporidiosis in other vertebrates. In: Cacciò S.M., Widmer G., editors. Cryptosporidium: Parasite and Disease. 1st ed. Springer; Vienna, Austria: 2014. pp. 237–326.

Tzanidakis N., Sotiraki S., Claerebout E., Ehsan A., Voutzourakis N., Kostopoulou D., Stijn C., Vercruysse J., Geurden T. Occurrence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in sheep and goats reared under dairy husbandry systems in Greece. Parasite. 2014;21:45. doi: 10.1051/parasite/2014048. PubMed DOI PMC

Mi R., Wang X., Huang Y., Zhou P., Liu Y., Chen Y., Chen J., Zhu W., Chen Z. Prevalence and molecular characterization of Cryptosporidium in goats across four provincial level areas in China. PLoS ONE. 2014;9:e111164. doi: 10.1371/journal.pone.0111164. PubMed DOI PMC

Wang R., Li G., Cui B., Huang J., Cui Z., Zhang S., Dong H., Yue D., Zhang L., Ning C., et al. Prevalence, molecular characterization and zoonotic potential of Cryptosporidium spp. in goats in Henan and Chongqing, China. Exp. Parasitol. 2014;142:11–16. doi: 10.1016/j.exppara.2014.04.001. PubMed DOI

Li P., Cai J., Cai M., Wu W., Li C., Lei M., Xu H., Feng L., Ma J., Feng Y., et al. Distribution of Cryptosporidium species in Tibetan sheep and yaks in Qinghai, China. Vet. Parasitol. 2016;215:58–62. doi: 10.1016/j.vetpar.2015.11.009. PubMed DOI

Kotková M., Němejc K., Sak B., Hanzal V., Květoňová D., Hlásková L., Čondlová S., McEvoy J., Kváč M. Cryptosporidium ubiquitum, C. muris and Cryptosporidium deer genotype in wild cervids and caprines in the Czech Republic. Folia Parasitol. 2016;63:003. doi: 10.14411/fp.2016.003. PubMed DOI

Qi M., Luo N., Wang H., Yu F., Wang R., Huang J., Zhang L. Zoonotic Cryptosporidium spp. and Enterocytozoon bieneusi in pet chinchillas (Chinchilla lanigera) in China. Parasitol. Int. 2015;64:339–341. doi: 10.1016/j.parint.2015.05.007. PubMed DOI

Kubota R., Matsubara K., Tamukai K., Ike K., Tokiwa T. Molecular and histopathological features of Cryptosporidium ubiquitum infection in imported chinchillas Chinchilla lanigera in Japan. Parasitol. Int. 2019;68:9–13. doi: 10.1016/j.parint.2018.09.002. PubMed DOI

Chen L., Hu S.H., Jiang W., Zhao J.G., Li N., Guo Y.Q., Liao C.H., Han Q., Feng Y.Y., Xiao L.H. Cryptosporidium parvum and Cryptosporidium hominis subtypes in crab-eating macaques. Parasit. Vectors. 2019;12:350. doi: 10.1186/s13071-019-3604-7. PubMed DOI PMC

Prediger J., Horčičková M., Hofmannová L., Sak B., Ferrari N., Mazzamuto M.V., Romeo C., Wauters L.A., McEvoy J., Kváč M. Native and introduced squirrels in Italy host different Cryptosporidium spp. Eur. J. Protistol. 2017;61:64–75. doi: 10.1016/j.ejop.2017.09.007. PubMed DOI

Vetterling J.M., Jervis H.R., Merrill T.G., Sprinz H. Cryptosporidium wrairi sp. n. from the guinea pig Cavia porcellus, with an emendation of the genus. J. Protozool. 1971;18:243–247. doi: 10.1111/j.1550-7408.1971.tb03315.x. PubMed DOI

Robertson L.J., Björkman C., Axén C., Fayer R. Cryptosporidiosis in Farmed Animals. In: Cacciò S.M., Widmer G., editors. Cryptosporidium: Parasite and Disease. Springer; Vienna, Austria: 2014. pp. 149–236.

Kváč M., Vlnatá G., Ježková J., Horčičková M., Konečný R., Hlásková L., McEvoy J., Sak B. Cryptosporidium occultus sp. n. (Apicomplexa: Cryptosporidiidae) in rats. Eur. J. Protistol. 2018;63:96–104. doi: 10.1016/j.ejop.2018.02.001. PubMed DOI

Fayer R., Santín M., Xiao L. Cryptosporidium bovis n. sp. (Apicomplexa: Cryptosporidiidae) in cattle (Bos taurus) J. Parasitol. 2005;91:624–629. doi: 10.1645/GE-3435. PubMed DOI

Ryan U.M., Monis P., Enemark H.L., Sulaiman I., Samarasinghe B., Read C., Buddle R., Robertson I., Zhou L., Thompson R.C.A., et al. Cryptosporidium suis n. sp. (Apicomplexa: Cryptosporidiidae) in pigs (Sus scrofa) J. Parasitol. 2004;90:769–773. doi: 10.1645/GE-202R1. PubMed DOI

Kváč M., Kestřánová M., Pinková M., Květoňová D., Kalinová J., Wagnerová P., Kotková M., Vítovec J., Ditrich O., McEvoy J., et al. Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa) Vet. Parasitol. 2013;191:218–227. doi: 10.1016/j.vetpar.2012.09.005. PubMed DOI PMC

Power M.L., Ryan U.M. A new species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) from eastern grey kangaroos (Macropus giganteus) J. Parasitol. 2008;94:1114–1117. doi: 10.1645/GE-1508.1. PubMed DOI

Kváč M., Květoňová D., Sak B., Ditrich O. Cryptosporidium pig genotype II in immunocompetent man. Emerg. Infect. Dis. 2009;15:982–983. doi: 10.3201/eid1506.071621. PubMed DOI PMC

Khan S.M., Debnath C., Pramanik A.K., Xiao L., Nozaki T., Ganguly S. Molecular characterization and assessment of zoonotic transmission of Cryptosporidium from dairy cattle in West Bengal, India. Vet. Parasitol. 2010;171:41–47. doi: 10.1016/j.vetpar.2010.03.008. PubMed DOI

Navarr-I-Martinez L., da Silva A.J., Bornay-Llinares F.J., Moura I.N.S., del Aguila C., Oleaga A., Pieniazek N.J. Detection and molecular characterization of Cryptosporidium bovis-like isolate from a newborn lamb in Spain. J. Parasitol. 2007;93:1536–1538. doi: 10.1645/GE-1116.1. PubMed DOI

Ren X., Zhao J., Zhang L., Ning C., Jian F., Wang R., Lv C., Wang Q., Arrowood M.J., Xiao L. Cryptosporidium tyzzeri n. sp. (Apicomplexa: Cryptosporidiidae) in domestic mice (Mus musculus) Exp. Parasitol. 2012;130:274–281. doi: 10.1016/j.exppara.2011.07.012. PubMed DOI

Tzipori S., Smith M., Halpin C., Angus K.W., Sherwood D., Campbell I. Experimental Cryptosporidiosis in Calves—Clinical Manifestations and Pathological Findings. Vet. Rec. 1983;112:116–120. doi: 10.1136/vr.112.6.116. PubMed DOI

Kváč M., Hofmannová L., Hlásková L., Květoňová D., Vítovec J., McEvoy J., Sak B. Cryptosporidium erinacei n. sp. (Apicomplexa: Cryptosporidiidae) in hedgehogs. Vet. Parasitol. 2014;201:9–17. doi: 10.1016/j.vetpar.2014.01.014. PubMed DOI

Deming C., Greiner E., Uhl E.W. Prevalence of Cryptosporidium infection and characteristics of oocyst shedding in a breeding colony of leopard geckos (Eublepharis macularius) J. Zoo Wildl. Med. 2008;39:600–607. doi: 10.1638/2006-016.1. PubMed DOI

Tzipori S., Smith M., Makin T., Halpin C. Enterocolitis in piglets caused by Cryptosporidium sp. purified from calf faeces. Vet. Parasitol. 1982;11:121–126. doi: 10.1016/0304-4017(82)90033-4. PubMed DOI

Čondlová S., Horčičková M., Sak B., Květoňová D., Hlásková L., Konečný R., Stanko M., McEvoy J., Kváč M. Cryptosporidium apodemi sp. n. and Cryptosporidium ditrichi sp. n. (Apicomplexa: Cryptosporidiidae) in Apodemus spp. Eur. J. Protistol. 2018;63:1–12. doi: 10.1016/j.ejop.2017.12.006. PubMed DOI

Umemiya R., Fukuda M., Fujisaki K., Matsui T. Electron microscopic observation of the invasion process of Cryptosporidium parvum in severe combined immunodeficiency mice. J. Parasitol. 2005;91:1034–1039. doi: 10.1645/GE-508R.1. PubMed DOI

Borowski H., Thompson R.C., Armstrong T., Clode P.L. Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system. Parasitology. 2010;137:13–26. doi: 10.1017/S0031182009990837. PubMed DOI

Fayer R., Santín M., Macarisin D. Cryptosporidium ubiquitum n. sp. in animals and humans. Vet. Parasitol. 2010;172:23–32. doi: 10.1016/j.vetpar.2010.04.028. PubMed DOI

Hofmannová L., Hauptman K., Huclová K., Květoňová D., Sak B., Kváč M. Cryptosporidium erinacei and C. parvum in a group of overwintering hedgehogs. Eur. J. Protistol. 2016;56:15–20. doi: 10.1016/j.ejop.2016.05.002. PubMed DOI

Holubová N., Tůmová L., Sak B., Hejzlerová A., Konečný R., McEvoy J., Kváč M. Description of Cryptosporidium ornithophilus sp. n. (Apicomplexa: Cryptosporidiidae) as a new species and diversity in farmed ostriches. Parasit. Vectors. 2020;13:340. doi: 10.1186/s13071-020-04191-2. PubMed DOI PMC

Lopez-Osorio S., Chaparro-Gutierrez J.J., Gomez-Osorio L.M. Overview of Poultry Eimeria Life Cycle and Host-Parasite Interactions. Front. Vet. Sci. 2020;7:384. doi: 10.3389/fvets.2020.00384. PubMed DOI PMC

Koehler A.V., Šlapeta J. Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection. In: Mead J.R., Arrowood M.J., editors. Cryptosporidium Methods and Protocols. Volume 2052. Springer Science+Business Media, LLC; New York, NY, USA: 2019. pp. 117–127. Methods in Molecular Biology. PubMed

Qari S.H., Goldman I.F., Pieniazek N.J., Collins W.E., Lal A.A. Blood and sporozoite stage-specific small subunit ribosomal RNA-encoding genes of the human malaria parasite Plasmodium vivax. Gene. 1994;150:43–49. doi: 10.1016/0378-1119(94)90855-9. PubMed DOI

Tang Y., Li N., Song M., Roellig D.M., Feng Y., Xiao L. Development of a multilocus sequence typing tool for high-resolution subtyping and genetic structure characterization of Cryptosporidium ubiquitum. Infect. Genet. Evol. 2016;45:256–261. doi: 10.1016/j.meegid.2016.09.011. PubMed DOI PMC

El-Sherry S., Ogedengbe M.E., Hafeez M.A., Barta J.R. Divergent nuclear 18S rDNA paralogs in a turkey coccidium, Eimeria meleagrimitis, complicate molecular systematics and identification. Int. J. Parasitol. 2013;43:679–685. doi: 10.1016/j.ijpara.2013.03.005. PubMed DOI

Le Blancq S.M., Khramtsov N.V., Zamani F., Upton S.J., Wu T.W. Ribosomal RNA gene organization in Cryptosporidium parvum. Mol. Biochem. Parasitol. 1997;90:463–478. doi: 10.1016/S0166-6851(97)00181-3. PubMed DOI

Nishimoto Y., Arisue N., Kawai S., Escalante A.A., Horii T., Tanabe K., Hashimoto T. Evolution and phylogeny of the heterogeneous cytosolic SSU rRNA genes in the genus Plasmodium. Mol. Phylogenet. Evol. 2008;47:45–53. doi: 10.1016/j.ympev.2008.01.031. PubMed DOI

Stenger B.L., Clark M.E., Kváč M., Khan E., Giddings C.W., Dyer N.W., Schultz J.L., McEvoy J.M. Highly divergent 18S rRNA gene paralogs in a Cryptosporidium genotype from eastern chipmunks (Tamias striatus) Infect. Genet. Evol. 2015;32:113–123. doi: 10.1016/j.meegid.2015.03.003. PubMed DOI PMC

Lindsay D.S., Upton S.J., Owens D.S., Morgan U.M., Mead J.R., Blagburn B.L. Cryptosporidium andersoni n. sp. (Apicomplexa: Cryptosporiidae) from cattle, Bos taurus. J. Eukaryot. Microbiol. 2000;47:91–95. doi: 10.1111/j.1550-7408.2000.tb00016.x. PubMed DOI

Holubová N., Sak B., Horčičková M., Hlásková L., Květoňová D., Menchaca S., McEvoy J., Kváč M. Cryptosporidium avium n. sp. (Apicomplexa: Cryptosporidiidae) in birds. Parasitol. Res. 2016;115:2243–2251. doi: 10.1007/s00436-016-4967-8. PubMed DOI PMC

Current W.L., Upton S.J., Haynes T.B. The life cycle of Cryptosporidium baileyi n. sp. (Apicomplexa, Cryptosporidiidae) infecting chickens. J. Protozool. 1986;33:289–296. doi: 10.1111/j.1550-7408.1986.tb05608.x. PubMed DOI

Lindsay D.S., Blagburn B.L., Sundermann C.A. Morphometric Comparison of the Oocysts of Cryptosporidium meleagridis and Cryptosporidium baileyi from Birds. Proc. Helminthol. Soc. Wash. 1989;56:91–92.

Bolland S.J., Zahedi A., Oskam C., Murphy B., Ryan U. Cryptosporidium bollandi n. sp. (Apicomplexa: Cryptosporidiiae) from angelfish (Pterophyllum scalare) and Oscar fish (Astronotus ocellatus) Exp. Parasitol. 2020;217:107956. doi: 10.1016/j.exppara.2020.107956. PubMed DOI

Fayer R., Trout J.M., Xiao L., Morgan U.M., Lai A.A., Dubey J.P. Cryptosporidium canis n. sp. from domestic dogs. J. Parasitol. 2001;87:1415–1422. doi: 10.1645/0022-3395(2001)087[1415:CCNSFD]2.0.CO;2. PubMed DOI

Paperna I., Vilenkin M. Cryptosporidiosis in the gourami Trichogaster leeri: Description of a new species and a proposal for a new genus, Piscicryptosporidium, for species infecting fish. Dis. Aquat. Organ. 1996;27:95–101. doi: 10.3354/dao027095. DOI

Robinson G., Wright S., Elwin K., Hadfield S.J., Katzer F., Bartley P.M., Hunter P.R., Nath M., Innes E.A., Chalmers R.M. Re-description of Cryptosporidium cuniculus Inman and Takeuchi, 1979 (Apicomplexa: Cryptosporidiidae): Morphology, biology and phylogeny. Int. J. Parasitol. 2010;40:1539–1548. doi: 10.1016/j.ijpara.2010.05.010. PubMed DOI

Ježková J., Horčičková M., Hlásková L., Sak B., Květoňová D., Novák J., Hofmannová L., McEvoy J., Kváč M. Cryptosporidium testudinis sp. n., Cryptosporidium ducismarci Traversa, 2010 and Cryptosporidium tortoise genotype III (Apicomplexa: Cryptosporidiidae) in tortoises. Folia Parasitol. 2016;63:035. doi: 10.14411/fp.2016.035. PubMed DOI

Ryan U.M., Power M., Xiao L. Cryptosporidium fayeri n. sp. (Apicomplexa: Cryptosporidiidae) from the Red Kangaroo (Macropus rufus) J. Eukaryot. Microbiol. 2008;55:22–26. doi: 10.1111/j.1550-7408.2007.00299.x. PubMed DOI

Iseki M. Cryptosporidium felis sp. n. (protozoa: Eimeriorina) from the domestic cat. Jap. J. Protozool. 1979;28:285–307.

Jirků M., Valigurová A., Koudela B., Křížek J., Modrý D., Šlapeta J. New species of Cryptosporidium Tyzzer, 1907 (Apicomplexa) from amphibian host: Morphology, biology and phylogeny. Folia Parasitol. (Praha) 2008;55:81–94. doi: 10.14411/fp.2008.011. PubMed DOI

Ryan U.M., Xiao L., Read C., Sulaiman I.M., Monis P., Lal A.A., Fayer R., Pavlásek I. A redescription of Cryptosporidium galli Pavlásek, 1999 (Apicomplexa: Cryptosporidiidae) from birds. J. Parasitol. 2003;89:809–813. doi: 10.1645/GE-74RI. PubMed DOI

Morgan-Ryan U.M., Fall A., Ward L.A., Hijjawi N., Sulaiman I., Fayer R., Thompson R.C., Olson M., Lal A., Xiao L. Cryptosporidium hominis n. sp. (Apicomplexa: Cryptosporidiidae) from Homo sapiens. J. Eukaryot. Microbiol. 2002;49:433–440. doi: 10.1111/j.1550-7408.2002.tb00224.x. PubMed DOI

Slavin D. Cryptosporidium meleagridis (sp. nov.) J. Comp. Pathol. 1955;65:262–266. doi: 10.1016/S0368-1742(55)80025-2. PubMed DOI

Alvarez-Pellitero P., Sitja-Bobadilla A. Cryptosporidium molnari n. sp. (Apicomplexa: Cryptosporidiidae) infecting two marine fish species, Sparus aurata L. and Dicentrarchus labrax L. Int. J. Parasitol. 2002;32:1007–1021. doi: 10.1016/S0020-7519(02)00058-9. PubMed DOI

Tyzzer E.E. An extracellular coccidium, Cryptosporidium muris (gen. et sp. nov.) of the gastric glands of the common mouse. J. Med. Res. 1910;23:487–509. PubMed PMC

Hoover D.M., Hoerr F.J., Carlton W.W., Hinsman E.J., Ferguson H.W. Enteric Cryptosporidiosis in a Naso Tang, Naso-Lituratus Bloch and Schneider. J. Fish Dis. 1981;4:425–428. doi: 10.1111/j.1365-2761.1981.tb01152.x. DOI

Upton S.J., Current W.L. The species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) infecting mammals. J. Parasitol. 1985;71:625–629. doi: 10.2307/3281435. PubMed DOI

Kváč M., Havrdová N., Hlásková L., Daňková T., Kanděra J., Ježková J., Vítovec J., Sak B., Ortega Y., Xiao L., et al. Cryptosporidium proliferans n. sp. (Apicomplexa: Cryptosporidiidae): Molecular and biological evidence of cryptic species within gastric Cryptosporidium of mammals. PLoS ONE. 2016;11:e0147090. doi: 10.1371/journal.pone.0147090. PubMed DOI PMC

Li X., Pereira M., Larsen R., Xiao C., Phillips R., Striby K., McCowan B., Atwill E.R. Cryptosporidium rubeyi n. sp. (Apicomplexa: Cryptosporidiidae) in multiple Spermophilus ground squirrel species. Int. J. Parasitol. Parasites Wildl. 2015;4:343–350. doi: 10.1016/j.ijppaw.2015.08.005. PubMed DOI PMC

Fayer R., Santín M., Trout J.M. Cryptosporidium ryanae n. sp. (Apicomplexa: Cryptosporidiidae) in cattle (Bos taurus) Vet. Parasitol. 2008;156:191–198. doi: 10.1016/j.vetpar.2008.05.024. PubMed DOI

Alvarez-Pellitero P., Quiroga M.I., Sitja-Bobadilla A., Redondo M.J., Palenzuela O., Padros F., Vazquez S., Nieto J.M. Cryptosporidium scophthalmi n. sp (Apicomplexa: Cryptosporidiidae) from cultured turbot Scophthalmus maximus. Light and electron microscope description and histopathological study. Dis. Aquat. Organ. 2004;62:133–145. doi: 10.3354/dao062133. PubMed DOI

Levine N.D. Some corrections of coccidian (Apicomplexa: Protozoa) nomenclature. J. Parasitol. 1980;66:830–834. doi: 10.2307/3280679. PubMed DOI

Pavlásek I., Lávisková M., Horák P., Král J., Král B. Cryptosporidium varanii n.sp. (Apicomplexa: Cryptosporidiidae) in Emerald monitor (Varanus prasinus Schlegal, 1893) in captivity in Prague zoo. Gazella. 1995;22:99–108.

Elwin K., Hadfield S.J., Robinson G., Crouch N.D., Chalmers R.M. Cryptosporidium viatorum n. sp. (Apicomplexa: Cryptosporidiidae) among travellers returning to Great Britain from the Indian subcontinent, 2007–2011. Int. J. Parasitol. 2012;42:675–682. doi: 10.1016/j.ijpara.2012.04.016. PubMed DOI

Fayer R., Santín M. Cryptosporidium xiaoi n. sp. (Apicomplexa: Cryptosporidiidae) in sheep (Ovis aries) Vet. Parasitol. 2009;164:192–200. doi: 10.1016/j.vetpar.2009.05.011. PubMed DOI

Cryptosporidium mortiferum n. sp. (Apicomplexa: Cryptosporidiidae), the species causing lethal cryptosporidiosis in Eurasian red squirrels (Sciurus vulgaris)

Parasitic Protists: Diversity of Adaptations to a Parasitic Lifestyle

High Occurrence of Zoonotic Subtypes of Cryptosporidiumparvum in Cypriot Dairy Farms

Cryptosporidium sciurinum n. sp. (Apicomplexa: Cryptosporidiidae) in Eurasian Red Squirrels (Sciurus vulgaris)