The nutria was introduced to Europe from South America and kept for the fur industry. This semiaquatic rodent became a well-established species in the Czech Republic; however, it still poses a significant threat to the native fauna, not only as a natural competitor but also as a vector of non-indigenous parasites. Our research aimed to investigate the diversity of endoparasitic helminths in nutria, with a particular focus on assessing the risk posed by helminth species with zoonotic potential. A total of 46 nutria cadavers were collected at 8 locations in the Morava River basin and examined using standard parasitological post-mortem procedures. Additionally, coprological and molecular methods were used to identify the parasites. The presence of 6 helminth species was revealed. The highest prevalence was observed for Strongyloides myopotami (78.3%) and Trichuris myocastoris (37.0%), both of which are host-specific nematodes of nutria. Only 2 trematode taxa were recorded (Echinostoma sp. and a representative of the family Psilostomidae). The presence of alveolar hydatid cysts of Echinococcus multilocularis in the livers of 5 nutria specimens was also recorded. Herein, we provide novel molecular data for each parasite species collected, which is valuable for future phylogenetic analyses. Our findings also demonstrate that nutria in the Czech Republic serve as a carrier of helminths with zoonotic potential, particularly E. multilocularis and S. myopotami. Although the nutria is a relatively new species in local fauna, its synanthropic behaviour raises concerns about potential threats to human health, underscoring the importance of exercising caution when handling these animals.

Department of Botany and Zoology Faculty of Science Masaryk University Brno Czech Republic

Department of Food Technology Faculty of AgriSciences Mendel University in Brno Brno Czech Republic

Department of Forest Protection and Wildlife Management Faculty of Forestry and Wood Technology Mendel University in Brno Brno Czech Republic

Department of Zoology Faculty of Natural Sciences Comenius University in Bratislava Bratislava Slovakia

Forestry and Game Management Research Institute Jíloviště Czech Republic

Institute of Vertebrate Biology Czech Academy of Sciences Brno Czech Republic

Zobrazit více v PubMed

Anděra M (2011) Current distributional status of rodents in the Czech Republic (Rodentia). Lynx 42, 5–82.

AOPK ČR (2023) Nálezová databáze ochrany přírody. Retrieved from https://portal.nature.cz/ (accessed 2 September 2023).

Atkinson IAE (1996) Introductions of wildlife as a cause of species extinctions. Wildlife Biology 2, 135–141.

Atopkin DM (2011) Genetic characterization of the PubMed

Babero BB and Lee JW (1961) Studies on the helminths of nutria, PubMed

Barratt JlN, Lane M, Talundzic E, Richins T, Robertson G, Formenti F, Pritt B, Verocai G, De Souza JN, Soares NM, Traub R, Buonfrate D and Bradbury RS (2019) A global genotyping survey of PubMed PMC

Benati D, Moraes MFD, Hoppe EGL, Tebaldi JH, Júnior IAN and da Costa Freitaz FL (2017) Helminths of the Nutria

Benovics M, Reslová N, Škorpíková L, Seidlová L and Mikulka O (2022) Helminth fauna of the Eurasian beaver in the Czech Republic with remarks on the genetic diversity of specialist PubMed

Bounds DL, Sherfy MH and Mollett TA (2003) Nutria. In Feldhamer GA, Thompson BC and Chapman JA (eds), Wild Mammals of North America: Biology Management and Conservation. Baltimore, MD: The Johns Hopkins University Press, pp. 1119–1147.

Bowles J and Mcmanus DP (1993) Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP method. Molecular and Biochemical Parasitology 57, 231–240. PubMed

Bray RA, Gibson D and Jones A (2008) Keys to the Trematoda: Volume 3. Wallingford, UK: CABI Publishing; & London, UK: The Natural History Museum.

Britton JR (2012) Introduced parasites in food webs: new species, shifting structures? Trends in Ecology and Evolution 28, 93–99. PubMed

Bush AO, Lafferty KD, Lotzs JM and Shostakll AW (1997) Parasitology meets ecology on its own terms: Margolis PubMed

Choe S, Lee D, Park H, Oh M, Jeon KH and Eom SK (2014) PubMed PMC

Czech Statistical Office (2024)

Dikmans G (1937) A note on the members of the nematode genus

Dunn AM (2009) Parasites and biological invasions. Advances in Parasitology 68, 161–184. PubMed

Eckert J, Conraths FJ and Rackmann K (2000) Echinococcosis: an emerging or reemerging zoonosis? International Journal for Parasitology: Parasites and Wildlife 30, 1283–1294. PubMed

El-Kouba MMAN, Marques SMT, Pilati C and Hamann W (2009) Presence of PubMed

ESFA (2021) Annual assessment of PubMed PMC

European Commission (2022) Commission implementing regulation (EU) 2016/1141 of 13 July 2016 adopting a list of invasive alien species of Union concern pursuant to regulation (EU) No 1143/2014 of the European Parliament and of the Council. Official Journal of the European Union, L 189, 1–9.

Evers D, Sasser CE, Gosselink JG, Fuller DA and Visser JM (1998) The impact of vertebrate herbivores on wetland vegetation in Atchafalaya Bay, Louisiana. Estuaries 21, 1–13.

Floyd RM, Rogers AD, Lambshead PJD and Smith CR (2005) Nematode-specific PCR primers for the 18S small subunit rRNA gene. Molecular Ecology Notes 5, 611–612.

Foreyt WJ (2002) Veterinary Parasitology Reference Manual, 5th Edn, Hoboken, USA: Wiley-Blackwell.

Fugassa MH (2020) Updated checklist of helminths found in terrestrial mammals of Argentine Patagonia. Journal of Helminthology 94, 1–56. PubMed

Genovesi P (2009) Species accounts of 100 of the most invasive alien species in Europe,

Georgiev B, Biserkov V and Genov T (1986) In toto staining method for cestodes with iron acetocarmine. Helminthologia 23, 279–291.

Gethöffer F and Siebert U (2020) Current knowledge of the Neozoa Nutria and Muskrat in Europe and their environmental impacts. Journal of Wildlife and Biodiversity 4, 1–12.

Ghasemikhah R, Mirhendi H, Kia EB, Mowlavi G, Sarmadian H, Meshgi B, Golestan B and Mobedi I (2011) Morphological and morphometrical description of PubMed PMC

Gibson DI, Jones A and Bray RA (2002) Keys to the Trematoda: Volume 1. Wallingford, UK: CABI Publishing; & London, UK: The Natural History Museum.

Goodfellow M, Shaw S and Morgan E (2006) Imported disease of dogs and cats exotic to Ireland: PubMed PMC

Grace JB and Ford MA (1996) The potential impact of herbivores on the susceptibility of the marsh plant

Hartel KS, Spittler H, Doering H, Winkelmann J, Hoerauf A and Reiter-Owona I (2004) The function of wild nutria (

Howerth EW, Reeves AJ, McElveen MR and Austin FW (1994) Survey for selected diseases in nutria ( PubMed

Issia L, Pietrokovsky S, Sousa-Figueiredo J, Stothard JR and Wisnivesky-Colli C (2009) PubMed

Janovsky M, Bacciarini L, Sager H, Grone A and Gottstein B (2002) PubMed

Ježková J, Limpouchová Z, Prediger J, Holubová N, Sak B, Konečný R, Květoňová D, Hlásková L, Rost M, McEvoy J, Rajský D, Feng Y and Kváč M (2021) PubMed PMC

Jirků-Pomajbíková K and Hůzová Z (2018) Coproscopic examination techniques. In Modrý D, Pafčo B, Petrželková JK and Hasegawa H (eds), Parasites of Apes. An Atlas of Coproscopic Diagnostics. Frankfurt: Edition Chimaira, pp. 22–28.

Jones A, Bray RA and Gibson DI (2005) Keys to the Trematoda: Volume 2. Wallingford, UK: CABI Publishing; & London, UK: The Natural History Museum.

Katoh K, Misawa K, Kuma K and Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30, 3059–3066. PubMed PMC

Kellnerová K, Holubová N, Jandová A, Vejčík A, McEvoy J, Sak B and Kváč M (2017) First description of PubMed

Kolářová L, Pavlásek I and Chalupský J (1996) PubMed

Kolářová L, Matějů J, Hozáková L, Stejskal F, Hrdý J, Kolářová H, Leissová M, Skála V and Dundr P (2017) Human alveolar echinococcosis and an overview of the occurrence of PubMed

Kostadinova A (2005) Family Psilostomidae Loos, 1900. In Jones A, Bray RA and Gibson DI (eds). Keys to the Trematoda: Volume 2. Wallingford, UK: CABI Publishing; & London, UK: The Natural History Museum, pp. 99–118.

Križman M, Švara T, Šoba B and Rataj AV (2022) Alveolar echinococcosis in nutria ( PubMed PMC

Lanave C, Preparata G, Saccone C and Serio G (1984) A new method for calculating evolutionary substitution rates. Journal of Molecular Evolution 20, 86–93. PubMed

Lewis DC and Ball SJ (1984)

Little MD (1965) Dermatitis in a human volunteer infected with PubMed

Lowe S, Browne M, Boudjelas S and De Poorter M (2000) 100 of the world's worst invasive alien species. A selection from the global invasive species database. The Invasive Species Specialist Group (ISSG) a specialist group of the Species Survival Commission (SSC) of the World Conservation Union (IUCN).

Martino PE, Radman N, Parrado E, Bautista E, Cisterna C, Silvestrini MP and Corba S (2012) Note on the occurrence of parasites of the wild nutria (

Martino PE, Stanchi NO, Silvestrini M, Brihuega B, Samartino L and Parrado E (2014) Seroprevalence for selected pathogens of zoonotic importance in wild nutria (

Martínek K, Kolářová L and Červený J (2001) PubMed

McManus PD, Zhang W, Li J and Bartley PB (2003) Echinococcosis. The Lancet 362, 1295–1304. PubMed

Ménard A, Agoulon A, L'Hostis M, Rondelaud D, Collard S and Chauvin A (2001) PubMed

Miura O, Kuris AM, Torchin ME, Hechinger RF, Dunham EJ and Chiba S (2005) Molecular-genetic analyses reveal cryptic species of trematodes in the intertidal gastropod, PubMed

Moravec F (2013) Parasitic Nematodes of Freshwater Fishes of Europe. Prague, CZ: Academia.

Nardoni S, Angelici MC, Mugnaini L and Mancianti F (2011) Prevalence of PubMed PMC

Nechybová S, Langrová I and Tůmová E (2018) Parasites of

Newton LA, Chilton NB, Beveridge I, Hoste H, Nansen P and Gasser RB (1998) Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA. Acta Tropica 69, 1–15. PubMed

Oksanen A, Siles-Lucas M, Karamon J, Possenti A, Conraths JF, Romig T, Wysocko P, Mannocci A, Mipatrini D, La Torre G, Boufana B and Casulli A (2016) The geographical distribution and prevalence of PubMed PMC

Olson PD, Cribb TH, Tkach VV, Bray RA and Littlewood DTJ (2003) Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33, 733–755. PubMed

Pavlásek I (1998) Actual situation and occurrence of

Prenter J, MacNeil C, Dick JTA and Dunn AM (2004) Roles of parasites in animal invasions. Trends in Ecology and Evolution 19, 385–390. PubMed

Rambaut A, Drummon AJ, Xie D, Baele G and Suchard MA (2018) Posterior summarization in Bayesian phylogenetic using Tracer 1.7. Systematic Biology 67, 901–904. PubMed PMC

Romig T (2009) PubMed

Romig T and Wassermann M (2024) PubMed PMC

Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA and Huelsenbeck JP (2012) Mrbayes 3.2: efficient Bayesian phylogenetic inference and model choice across large model space. Systematic Biology 61, 539–542. PubMed PMC

Rossin AM, Varela G and Timi TJ (2009)

Rózsa L, Reiczigel J and Majoros G (2000) Quantifying parasites in samples of hosts. Journal of Parasitology 86, 228–232. PubMed

Rylková K, Tůmová E, Brožová A, Jankovská I, Vadlejch J, Čadková Z, Frýdlová J, Peřinková P, Langrová I, Chodová D, Nechybová S and Scháňková Š (2015) Genetic and morphological characterization of PubMed

Santoro A, Santolamazza F, Cacciò SM, La Rosa G, Antolová D, Auer H, Bagrade G, Bandelj P, Basso W, Beck R, Citterio CV, Davidson RK, Deksne G, Frey CF, Fuglei E, Glawischnig W, Gottstein B, Harna J, Petersen HH and Karamon J (2024) Mitochondrial genetic diversity and phylogenetic relationships of PubMed

Scheuring W (1990) Examination of the intestinal parasite fauna of nutria (

Shaffer GP, Day JW, Hunter RG, Lane RR, Lundberg CJ, Wood WB and Kandalepas D (2015) System response, nutria herbivory, and vegetation recovery of a wetland receiving secondarily treated effluent in coastal Louisiana. Ecological Engineering 79, 120–131.

Sheather AL (1923) The detection of intestinal protozoa and mange parasites by a floatation technique. Journal of Comparative Pathology and Therapeutics 36, 266–275.

Šoba B, Gašperšič Š, Keše D and Kotar T (2020) Molecular Characterization of PubMed PMC

Sréter T, Széll Z, Egyed Z and Varga I (2003) PubMed PMC

Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22, 2688–2690. PubMed

Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analyses and post-analysis of large phylogenies. Bioinformatics 30, 1312–1313. PubMed PMC

Sutor A (2008) Dispersal of the alien raccoon dog

Szemethy L, Heltai M and Biró Z (2000) Effects of oral immunisation against rabies on the dynamics of the fox population. Magyar Állatorvosok Lapja 122, 612–617.

Umhang G, Richomme C, Boucher JM, Guedon G and Boué F (2013) Nutrias and muskrats as bioindicators for the presence of PubMed

Verweij JJ, Canales M, Polman K, Ziem J, Brienen EAT, Polderman AM and van Lieshout L (2009) Molecular diagnosis of PubMed

Woods CA, Contreras L, Willner-Chapman G and Whidden PH (1992) Myocastor coypus. Mammalian Species 398, 1–8.

Zanzani SA, Di Cerbo A, Gazzonis AL, Epis S, Invernizzi A, Tagliabue S and Manfredi MT (2016) Parasitic and bacterial infections of PubMed

First record of Baylisascaris procyonis in the wild invasive Northern raccoon (Procyon lotor) in the Czech Republic