Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25704490
DOI
10.1039/c5cc00530b
Knihovny.cz E-resources
- MeSH
- Aniline Compounds chemistry MeSH
- Benzylidene Compounds chemistry MeSH
- DNA-Binding Proteins chemistry MeSH
- DNA-Directed DNA Polymerase metabolism MeSH
- DNA chemistry MeSH
- Fluorescent Dyes chemistry MeSH
- Molecular Structure MeSH
- Nitriles chemistry MeSH
- Protein Binding MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 2-cyanoacetamide MeSH Browser
- Aniline Compounds MeSH
- Benzylidene Compounds MeSH
- DNA-Binding Proteins MeSH
- DNA-Directed DNA Polymerase MeSH
- DNA MeSH
- Fluorescent Dyes MeSH
- Nitriles MeSH
Viscosity-sensitive fluorophores, fluorescent molecular rotors based on aminobenzylidene-cyanoacetamide moiety, were tethered to 2'-deoxycytidine triphosphate via a propargylamine linker and incorporated into DNA by polymerases in primer extension, nicking enzyme amplification or PCR. DNA probes incorporating modified nucleosides show a light-up response upon binding to a protein.
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