Structural insight into the calcium ion modulated interdomain electron transfer in cellobiose dehydrogenase
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25862501
DOI
10.1016/j.febslet.2015.03.029
PII: S0014-5793(15)00207-0
Knihovny.cz E-resources
- Keywords
- Calcium effect, Cellobiose dehydrogenase, Electrostatic interaction, Flavocytochrome, Hydrogen/deuterium exchange, Interdomain electron transfer,
- MeSH
- Fungal Proteins chemistry MeSH
- Carbohydrate Dehydrogenases chemistry MeSH
- Sordariales enzymology MeSH
- Protein Structure, Tertiary MeSH
- Electron Transport physiology MeSH
- Calcium chemistry MeSH
- Structure-Activity Relationship MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- cellobiose-quinone oxidoreductase MeSH Browser
- Fungal Proteins MeSH
- Carbohydrate Dehydrogenases MeSH
- Calcium MeSH
Cellobiose dehydrogenase (CDH) from wood degrading fungi represents a subclass of oxidoreductases with unique properties. Consisting of two domains exhibiting interdomain electron transfer, this is the only known flavocytochrome involved in wood degradation. High resolution structures of the separated domains were solved, but the overall architecture of the intact protein and the exact interface of the two domains is unknown. Recently, it was shown that divalent cations modulate the activity of CDH and its pH optimum and a possible mechanism involving bridging of negative charges by calcium ions was proposed. Here we provide a structural explanation of this phenomenon confirming the interaction between negatively charged surface patches and calcium ions at the domain interface.
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