We studied malignant melanoma cell line Me45 and human ovarian carcinoma cell line SKOV-3 (resistant to cisplatin, adriamycin and diphtheria toxin), assessing their expression level of p53, HSP70 and glutathione S-transferase GST-π before and after chemotherapy with cisplatin. These proteins may be responsible for the occurrence of chemoresistance in cancer patients. To assess protein expression we used the immunocytochemical Avidin-Biotin-peroxidase Complex (ABC) method. Before application of chemotherapy, proteins p53, HSP70 and GST-π were present in 100 % of the examined melanoma cells. After the treatment, the intensity of the immunocytochemical reaction for p53 increased, whereas the intensity of immunocytochemical staining for HSP70 and GST-π decreased. In SKOV-3 cells, p53 and HSP70 were present in 100 % of the examined cells both prior to chemotherapy and after it. However, the intensity of the immunocytochemical reaction for p53 decreased, while that of HSP70 increased. As regards GST-π, only 5 % of all examined SKOV-3 cells revealed its expression before chemotherapy. Incubation with cisplatin caused an elevation in the number of ovarian cancer cells expressing GST-π up to 50 %. Moreover, the intensity of the immunocytochemical reaction for GST-π significantly increased.

2nd Department and Clinic of General and Oncological Surgery Wroclaw Medical University Wroclaw Poland

Department of Animal Developmental Biology University of Wroclaw Wroclaw Poland

Faculty of Medicine Wroclaw Medical University Wroclaw Poland

Student Scientific Circle of the Department of Medical Biochemistry Wroclaw Medical University Wroclaw Poland

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