Optimization of carvacrol, rosmarinic, oleanolic and ursolic acid extraction from oregano herbs (Origanum onites L., Origanum vulgare spp. hirtum and Origanum vulgare L.)
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
- Keywords
- HPLC, Origanum, carvacrol, extraction, oleanolic acid, rosmarinic acid, ursolic acid,
- MeSH
- Cinnamates isolation & purification MeSH
- Cymenes MeSH
- Depsides isolation & purification MeSH
- Origanum chemistry classification MeSH
- Oleanolic Acid isolation & purification MeSH
- Rosmarinic Acid MeSH
- Ursolic Acid MeSH
- Molecular Structure MeSH
- Monoterpenes isolation & purification MeSH
- Plant Extracts chemistry MeSH
- Solvents MeSH
- Triterpenes isolation & purification MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- carvacrol MeSH Browser
- Cinnamates MeSH
- Cymenes MeSH
- Depsides MeSH
- Oleanolic Acid MeSH
- Monoterpenes MeSH
- Plant Extracts MeSH
- Solvents MeSH
- Triterpenes MeSH
The aim of our study was to increase the extraction efficiency of carvacrol, rosmarinic, oleanolic and ursolic acid from the different species of oregano herbs (Origanum onites L., Origanum vulgare spp. hirtum and Origanum vulgare L.). Various extraction methods (ultrasound-assisted, heat-reflux, continuous stirring, maceration, percolation) and extraction conditions (different solvent, material:solvent ratio, extraction temperature, extraction time) were used, and the active substances were determined by HPLC. The lowest content of carvacrol, rosmarinic, oleanolic and ursolic acid was obtained by percolation. During heat-reflux extraction, the content of active substances depended on the solvent used: ethanol/non-aqueous solvent (glycerol or propylene glycol) mixture was more effective compared with ethanol alone. The results showed that for each species of oregano the most optimal extraction method should be selected to maximize the content of biologically active substances in the extracts.
b Department of Pharmacognosy Lithuanian University of Health Sciences Kaunas Lithuania
c Department of Drug Chemistry Lithuanian University of Health Sciences Kaunas Lithuania
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