Pseudomonas moraviensis subsp. stanleyae, a bacterial endophyte of hyperaccumulator Stanleya pinnata, is capable of efficient selenite reduction to elemental selenium under aerobic conditions
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25968181
DOI
10.1111/jam.12842
Knihovny.cz E-resources
- Keywords
- Pseudomonas moraviensis, Stanleya pinnata, aerobic selenite reduction, elemental selenium nanoparticles, microchip capillary electrophoresis, multi-locus sequence analysis,
- MeSH
- Aerobiosis MeSH
- Biodegradation, Environmental MeSH
- Brassicaceae metabolism microbiology MeSH
- Endophytes classification genetics isolation & purification metabolism MeSH
- Selenious Acid metabolism MeSH
- Pseudomonas classification genetics isolation & purification metabolism MeSH
- Selenium metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Selenious Acid MeSH
- Selenium MeSH
AIMS: To identify bacteria with high selenium tolerance and reduction capacity for bioremediation of wastewater and nanoselenium particle production. METHODS AND RESULTS: A bacterial endophyte was isolated from the selenium hyperaccumulator Stanleya pinnata (Brassicaceae) growing on seleniferous soils in Colorado, USA. Based on fatty acid methyl ester analysis and multi-locus sequence analysis (MLSA) using 16S rRNA, gyrB, rpoB and rpoD genes, the isolate was identified as a subspecies of Pseudomonas moraviensis (97.3% nucleotide identity) and named P. moraviensis stanleyae. The isolate exhibited extreme tolerance to SeO3(2-) (up to 120 mmol l(-1)) and SeO4(2-) (>150 mmol l(-1)). Selenium oxyanion removal from growth medium was measured by microchip capillary electrophoresis (detection limit 95 nmol l(-1) for SeO3(2-) and 13 nmol l(-1) for SeO4(2-)). Within 48 h, P. moraviensis stanleyae aerobically reduced SeO3(2-) to red Se(0) from 10 mmol l(-1) to below the detection limit (removal rate 0.27 mmol h(-1) at 30 °C); anaerobic SeO3(2-) removal was slower. No SeO4(2-) removal was observed. Pseudomonas moraviensis stanleyae stimulated the growth of crop species Brassica juncea by 70% with no significant effect on Se accumulation. CONCLUSIONS: Pseudomonas moraviensis stanleyae can tolerate extreme levels of selenate and selenite and can deplete high levels of selenite under aerobic and anaerobic conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: Pseudomonas moraviensis subsp. stanleyae may be useful for stimulating plant growth and for the treatment of Se-laden wastewater.
Biology Department Colorado State University Fort Collins CO USA
Chemistry Department Colorado State University Fort Collins CO USA
UNESCO IHE Institute for Water Education Delft The Netherlands
Université Paris Est Laboratoire Géomatériaux et Environnement UPEM Marne la Vallée Cedex 2 France
References provided by Crossref.org
