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Time-resolved microspectrofluorometry and fluorescence imaging techniques: study of porphyrin-mediated cellular uptake of oligonucleotides
P Praus, E Kocisova, P Mojzes, J Stepanek, O Seksek, F Sureau, PY Turpin
Language English Country United States
Document type Review
NLK
Medline Complete (EBSCOhost)
from 2006-04-01 to 1 year ago
Wiley Online Library (archiv)
from 1997-01-01 to 2012-12-31
- MeSH
- Oligonucleotides, Antisense chemistry MeSH
- Cell Nucleus metabolism MeSH
- 3T3 Cells MeSH
- Time Factors MeSH
- Financing, Organized MeSH
- Fluorescence MeSH
- Microscopy, Fluorescence methods instrumentation MeSH
- Cations MeSH
- Microscopy, Confocal methods instrumentation MeSH
- Drug Delivery Systems MeSH
- Melanoma, Experimental MeSH
- Mice MeSH
- Oligonucleotides chemistry MeSH
- Porphyrins chemistry MeSH
- Sensitivity and Specificity MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Review MeSH
Time-resolved confocal microspectrofluorometry and fluorescence microscopy imaging were applied to monitor the cellular uptake of fluorescent-labeled oligonucleotides (ONs) delivered by a porphyrin molecule. The fate of porphyrin-ON complexes inside living cells has also been monitored. Due to intrinsic fluorescence of the porphyrin and sensitivity of its characteristics to microenvironment, multicomponent analysis of time-resolved fluorescence provides unique information about stability of the porphyrin-ON complexes, ON interactions with their target sequences, and ON and porphyrin distributions after delivery inside the cells. Time-resolved confocal microspectrofluorometry indeed delivers additional information compared with fluorescence confocal microscopy imaging widely employed to study ON uptake.
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- $a Time-resolved confocal microspectrofluorometry and fluorescence microscopy imaging were applied to monitor the cellular uptake of fluorescent-labeled oligonucleotides (ONs) delivered by a porphyrin molecule. The fate of porphyrin-ON complexes inside living cells has also been monitored. Due to intrinsic fluorescence of the porphyrin and sensitivity of its characteristics to microenvironment, multicomponent analysis of time-resolved fluorescence provides unique information about stability of the porphyrin-ON complexes, ON interactions with their target sequences, and ON and porphyrin distributions after delivery inside the cells. Time-resolved confocal microspectrofluorometry indeed delivers additional information compared with fluorescence confocal microscopy imaging widely employed to study ON uptake.
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