The aim of this study was a reliable intra-species discrimination and strain biodiversity in Oenococcus oeni populations of two different Aglianico wineries by molecular, biochemical, and physiological characterization. Pulsed field gel electrophoresis (PFGE) analysis revealed a high polymorphism related to the origin (winery) of strains, while differential display PCR (DD-PCR) allowed a further discrimination of strains from the same winery. Moreover, the heterogeneity of these natural populations was investigated by capillary electrophoresis and enzymatic assays. A variability related to a different surface charge distribution was observed among strains, linked to their origin. Malolactic activity study evidenced strain-specific differences in malic acid degradation, and then, only the presence of L(-)-malic acid in the medium induced the mle gene. This study provided evidences on the importance of intra-species biodiversity of malolactic bacterial populations in wine ecosystems, as each wine possess peculiar winemaking conditions and physical-chemical properties which make specific the bacterial survival and growth. This study highlighted a great biodiversity among O. oeni strains that can be also winery specific. Such biodiversity within a certain winery and winemaking area is important for selecting malolactic starters, and strain-specific trait identification is especially important to match individual strains to specific industrial process.

Dipartimento di Scienze Università degli Studi della Basilicata Viale dell'Ateneo Lucano 10 85100 Potenza Italy

Zobrazit více v PubMed

Appl Microbiol Biotechnol. 2010 Mar;86(2):681-91 PubMed

Microb Ecol. 2012 Jan;63(1):12-9 PubMed

Int J Food Microbiol. 2010 Feb 28;137(2-3):230-5 PubMed

J Food Sci. 2012 Oct;77(10):M579-85 PubMed

FEMS Microbiol Lett. 2001 Jan 1;194(1):33-7 PubMed

Food Microbiol. 2008 Oct;25(7):942-8 PubMed

Int J Food Microbiol. 2008 Mar 31;123(1-2):171-6 PubMed

J Appl Microbiol. 2010 Jan;108(1):285-98 PubMed

Nucleic Acids Res. 1987 Aug 11;15(15):5985-6005 PubMed

Appl Environ Microbiol. 1982 Feb;43(2):357-61 PubMed

Lett Appl Microbiol. 2008 Aug;47(2):134-9 PubMed

J Appl Microbiol. 2012 Nov;113(5):1087-96 PubMed

FEMS Microbiol Lett. 2002 Jun 4;211(2):155-9 PubMed

Syst Appl Microbiol. 2006 Jul;29(5):375-81 PubMed

Appl Environ Microbiol. 2009 Apr;75(7):2079-90 PubMed

Curr Microbiol. 2000 Jun;40(6):351-5 PubMed

Res Microbiol. 2006 Apr;157(3):267-74 PubMed

Appl Microbiol Biotechnol. 2011 Nov;92 (3):441-7 PubMed

FEMS Microbiol Lett. 2009 Jul;296(1):11-7 PubMed

Int J Food Microbiol. 2009 Nov 30;136(1):123-8 PubMed

Appl Environ Microbiol. 1996 Dec;62(12 ):4493-8 PubMed

Anal Chem. 2009 Jan 1;81(1):8-15 PubMed

Lett Appl Microbiol. 2010 Mar;50(3):327-34 PubMed

Int J Food Microbiol. 2008 Oct 31;127(3):241-5 PubMed

Meat Sci. 2008 Dec;80(4):1238-48 PubMed

FEMS Microbiol Lett. 2001 Aug 7;202(1):109-14 PubMed

Analyst. 2010 Jul;135(7):1636-44 PubMed

Appl Environ Microbiol. 2003 Oct;69(10):5826-32 PubMed

J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Feb 5;800(1-2):7-25 PubMed

Food Microbiol. 2012 Feb;29(1):113-20 PubMed

Syst Appl Microbiol. 2010 Dec;33(8):461-7 PubMed

Lett Appl Microbiol. 1998 Nov;27(5):243-6 PubMed

Biotechnology (N Y). 1993 Nov;11(11):1278-82 PubMed

Appl Environ Microbiol. 1984 Oct;48(4):900-1 PubMed

Curr Microbiol. 2011 Jun;62(6):1682-8 PubMed

Microbiol Mol Biol Rev. 2003 Mar;67(1):38-51, table of contents PubMed

J Bacteriol. 2008 Jan;190(2):564-70 PubMed

FEMS Microbiol Lett. 2004 Jan 15;230(1):53-61 PubMed

Biochim Biophys Acta. 2005 Nov 30;1717(2):118-24 PubMed

Int J Food Microbiol. 2005 Dec 15;105(3):463-70 PubMed

Appl Environ Microbiol. 1989 Aug;55(8):2010-2016 PubMed

Electrophoresis. 2002 Jul;23(13):1957-72 PubMed

J Biosci Bioeng. 2009 Sep;108(3):220-4 PubMed

Anal Chem. 1999 Dec 15;71(24):5465-9 PubMed