A novel satellite DNA sequence in the Peromyscus genome (PMSat): Evolution via copy number fluctuation
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26103000
DOI
10.1016/j.ympev.2015.06.008
PII: S1055-7903(15)00178-5
Knihovny.cz E-resources
- Keywords
- Copy number, Laser microdissection, Rodentia, Satellite DNA,
- MeSH
- Species Specificity MeSH
- Phylogeny MeSH
- Physical Chromosome Mapping MeSH
- Genome * MeSH
- Gene Dosage * MeSH
- Peromyscus genetics MeSH
- Evolution, Molecular * MeSH
- Molecular Sequence Data MeSH
- Computer Simulation MeSH
- Restriction Mapping MeSH
- DNA, Satellite genetics isolation & purification MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Sequence Alignment MeSH
- Blotting, Southern MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Satellite MeSH
Satellite DNAs (satDNA) are tandemly arrayed repeated sequences largely present in eukaryotic genomes, which play important roles in genome evolution and function, and therefore, their analysis is vital. Here, we describe the isolation of a novel satellite DNA family (PMSat) from the rodent Peromyscus eremicus (Cricetidae, Rodentia), which is located in pericentromeric regions and exhibits a typical satellite DNA genome organization. Orthologous PMSat sequences were isolated and characterized from three species belonging to Cricetidae: Cricetus cricetus, Phodopus sungorus and Microtus arvalis. In these species, PMSat is highly conserved, with the absence of fixed species-specific mutations. Strikingly, different numbers of copies of this sequence were found among the species, suggesting evolution by copy number fluctuation. Repeat units of PMSat were also found in the Peromyscus maniculatus bairdii BioProject, but our results suggest that these repeat units are from genome regions outside the pericentromere. The remarkably high evolutionary sequence conservation along with the preservation of a few numbers of copies of this sequence in the analyzed genomes may suggest functional significance but a different sequence nature/organization. Our data highlight that repeats are difficult to analyze due to the limited tools available to dissect genomes and the fact that assemblies do not cover regions of constitutive heterochromatin.
References provided by Crossref.org
Satellite DNA in Neotropical Deer Species
Sequence Analysis and FISH Mapping of Four Satellite DNA Families among Cervidae
GENBANK
GQ902036, KC351938, KC351939, KC351940, KC351941, KC351942, KC351943, KC351944, KC351945, KC351946, KC351947, KC351948, KC351949, KC351950, KC351951, KC351952, KC351953, KC351954
