Short-term stability of Borrelia garinii in cerebrospinal fluid
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26104540
DOI
10.1007/s12223-015-0408-9
PII: 10.1007/s12223-015-0408-9
Knihovny.cz E-zdroje
- MeSH
- Borrelia burgdorferi komplex fyziologie MeSH
- časové faktory MeSH
- lidé MeSH
- lymeská nemoc diagnóza mikrobiologie MeSH
- mikrobiální viabilita * MeSH
- mozkomíšní mok mikrobiologie MeSH
- odběr biologického vzorku metody MeSH
- teplota MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The aim of our study was to find out the optimal conditions for short-term storage of cerebrospinal fluid (CSF) samples for direct diagnosis of Lyme disease. A mixture of Borrelia-negative CSFs spiked with a defined amount of cultured Borrelia garinii was used. Borrelia stability was investigated over 7 days at four different temperatures [room temperature (RT), +4, -20 and -70 °C]. Quantitative changes in CSF Borrelia were measured by quantitative PCR (qPCR), and morphological changes in the spirochetes were observed by transmission electron microscopy (TEM). These qPCR results were statistically evaluated. We found +4 °C to be an optimal temperature for short-term storage of CSF samples intended for TEM observation. There was no significant difference between the temperatures tested in the average quantity of Borrelia measured by qPCR. On the contrary, electron optical diagnosis of frozen samples and samples stored at RT showed destructive morphological changes and decreased spirochete counts. Our results show that optimal conditions for the pre-analytical phase of investigation of one type of material can differ depending on the diagnostic method employed.
Zobrazit více v PubMed
Neuro Endocrinol Lett. 2011;32(4):491-5 PubMed
Folia Biol (Praha). 2003;49(1):40-8 PubMed
Clin Microbiol Infect. 2001 Sep;7(9):461-9 PubMed
Yale J Biol Med. 1984 Jul-Aug;57(4):521-5 PubMed
Eur J Neurol. 2006 Sep;13(9):913-22 PubMed
Folia Microbiol (Praha). 2014 Mar;59(2):115-25 PubMed
Clin Microbiol Infect. 2004 Dec;10(12):1108-32 PubMed
Transfusion. 1990 May;30(4):298-301 PubMed
J Clin Microbiol. 2002 Dec;40(12):4581-4 PubMed
J Clin Pathol. 2008 Feb;61(2):221-4 PubMed
Zentralbl Bakteriol. 1998 Mar;287(3):190-5 PubMed
Mol Diagn. 1998 Sep;3(3):131-141 PubMed
Przegl Epidemiol. 2013;67(1):35-9, 119-23 PubMed
Int J Dermatol. 2008 Oct;47(10):1004-10 PubMed
Yale J Biol Med. 1984 Jul-Aug;57(4):543-8 PubMed
Eur J Heart Fail. 2012 Jun;14(6):588-96 PubMed
J Infect Dis. 1990 Aug;162(2):557-9 PubMed
Am J Syph Gonorrhea Vener Dis. 1948 Jan;32(1):1-18 PubMed
