The actin family members, consisting of actin and actin-related proteins (ARPs), are essential components of chromatin remodeling complexes. ARP6, one of the nuclear ARPs, is part of the Snf-2-related CREB-binding protein activator protein (SRCAP) chromatin remodeling complex, which promotes the deposition of the histone variant H2A.Z into the chromatin. In this study, we showed that ARP6 influences the structure and the function of the nucleolus. ARP6 is localized in the central region of the nucleolus, and its knockdown induced a morphological change in the nucleolus. We also found that in the presence of high concentrations of glucose ARP6 contributed to the maintenance of active ribosomal DNA (rDNA) transcription by placing H2A.Z into the chromatin. In contrast, under starvation, ARP6 was required for cell survival through the repression of rDNA transcription independently of H2A.Z. These findings reveal novel pleiotropic roles for the actin family in nuclear organization and metabolic homeostasis.

Department of Biology of the Cell Nucleus Institute of Molecular Genetics of the Academy of Sciences of the Czech Republic v v i Vídeňská 1083 142 20 Prague Czech Republic

Department of Medical Cell Biology Institute of Molecular Embryology and Genetics Kumamoto University 2 2 1 Honjo Chuo ku Kumamoto 860 0811 Japan

Department of Medical Cell Biology Institute of Molecular Embryology and Genetics Kumamoto University 2 2 1 Honjo Chuo ku Kumamoto 860 0811 Japan; Core Research for Evolutional Science and Technology Japan Science and Technology Agency Tokyo 102 0076 Japan

Image Informatics and Computational Biology Unit Laboratory of Genetics National Institute on Aging National Institutes of Health 251 Bayview Boulevard Suite 100 Baltimore MD 21224 USA

Laboratory of Molecular Biology Graduate School of Agricultural Science Tohoku University Tsutsumidori Amamiyamachi 1 1 Aoka ku Sendai 981 8555 Japan

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