Aurora kinase A is essential for correct chromosome segregation in mouse zygote
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
26174602
DOI
10.1017/s0967199415000222
PII: S0967199415000222
Knihovny.cz E-resources
- Keywords
- Apoptosis, Aurora A, MLN8237, Mouse zygote, Spindle,
- MeSH
- Aurora Kinase A antagonists & inhibitors metabolism MeSH
- Azepines pharmacology MeSH
- Time-Lapse Imaging MeSH
- Microscopy, Fluorescence MeSH
- Phosphorylation drug effects MeSH
- Protein Kinase Inhibitors pharmacology MeSH
- Microscopy, Confocal MeSH
- Embryo Culture Techniques MeSH
- Mitosis drug effects physiology MeSH
- Mice, Inbred BALB C MeSH
- Mice, Inbred C57BL MeSH
- Pyrimidines pharmacology MeSH
- Chromosome Segregation drug effects physiology MeSH
- Animals MeSH
- Zygote drug effects physiology MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Aurora Kinase A MeSH
- Azepines MeSH
- Protein Kinase Inhibitors MeSH
- MLN 8237 MeSH Browser
- Pyrimidines MeSH
Aurora-A kinase (AURKA), a member of the serine/threonine protein kinase family, is involved in multiple steps of mitotic progression. It regulates centrosome maturation, mitotic spindle formation, and cytokinesis. While studied extensively in somatic cells, little information is known about AURKA in the early cleavage mouse embryo with respect to acentrosomal spindle assembly. In vitro experiments in which AURKA was inactivated with specific inhibitor MLN8237 during the early stages of embryogenesis documented gradual arrest in the cleavage ability of the mouse embryo. In the AURKA-inhibited 1-cell embryos, spindle formation and anaphase onset were delayed and chromosome segregation was defective. AURKA inhibition increased apoptosis during early embryonic development. In conclusion these data suggest that AURKA is essential for the correct chromosome segregation in the first mitosis as a prerequisite for normal later development after first cleavage.
Institute of Animal Physiology Slovak Academy of Sciences Kosice Slovakia
Institute of Animal Physiology Slovak Academy of Sciences Soltesovej 4 040 01 Kosice Slovakia
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