Aurora kinase A is essential for correct chromosome segregation in mouse zygote
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26174602
DOI
10.1017/s0967199415000222
PII: S0967199415000222
Knihovny.cz E-zdroje
- Klíčová slova
- Apoptosis, Aurora A, MLN8237, Mouse zygote, Spindle,
- MeSH
- aurora kinasa A antagonisté a inhibitory metabolismus MeSH
- azepiny farmakologie MeSH
- časosběrné zobrazování MeSH
- fluorescenční mikroskopie MeSH
- fosforylace účinky léků MeSH
- inhibitory proteinkinas farmakologie MeSH
- konfokální mikroskopie MeSH
- kultivace embrya MeSH
- mitóza účinky léků fyziologie MeSH
- myši inbrední BALB C MeSH
- myši inbrední C57BL MeSH
- pyrimidiny farmakologie MeSH
- segregace chromozomů účinky léků fyziologie MeSH
- zvířata MeSH
- zygota účinky léků fyziologie MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aurora kinasa A MeSH
- azepiny MeSH
- inhibitory proteinkinas MeSH
- MLN 8237 MeSH Prohlížeč
- pyrimidiny MeSH
Aurora-A kinase (AURKA), a member of the serine/threonine protein kinase family, is involved in multiple steps of mitotic progression. It regulates centrosome maturation, mitotic spindle formation, and cytokinesis. While studied extensively in somatic cells, little information is known about AURKA in the early cleavage mouse embryo with respect to acentrosomal spindle assembly. In vitro experiments in which AURKA was inactivated with specific inhibitor MLN8237 during the early stages of embryogenesis documented gradual arrest in the cleavage ability of the mouse embryo. In the AURKA-inhibited 1-cell embryos, spindle formation and anaphase onset were delayed and chromosome segregation was defective. AURKA inhibition increased apoptosis during early embryonic development. In conclusion these data suggest that AURKA is essential for the correct chromosome segregation in the first mitosis as a prerequisite for normal later development after first cleavage.
Institute of Animal Physiology Slovak Academy of Sciences Kosice Slovakia
Institute of Animal Physiology Slovak Academy of Sciences Soltesovej 4 040 01 Kosice Slovakia
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