Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latter were used to correct for variation in the first electrophoretic dimension and further improve protein coordinate assignment in 2D-gel electrophoresis. Data tables are supplied to demonstrate pI-value calibration and the effect on the assignment of protein spot coordinates.

Core Unit Proteomics Interdisciplinary Center for Clinical Research University of Münster Germany

Institute of Analytical Chemistry of the ASCR v v i Brno Czech Republic

See more in PubMed

M. Hanneken, K. Šlais, S. König pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using Amphoteric Azo Dyes EuPA Open Proteomics, 2015, http://dx.doi.org/10.1016/j.euprot.2015.03.003. PubMed PMC

Ackermann D., Wang W., Streipert B., Geib B., Grün L., König S. Comparative fluorescence two-dimensional gel electrophoresis using a gel strip sandwich assembly for the simultaneous on-gel generation of a reference protein spot grid Electrophoresis. 2012;33:1406–1410. PubMed

D. Ackermann, W. Wang, L. Grün, S. König Improved gel electrophoresis Patent application EP11167383.6, May 25, 2011; WO 2012/159769 A1 Nov. 29, 2012; Patent No. 12729346.2-1554 May 25, 2012, publication number EP 2 715 331.

Hanneken M., König S. Horizontal comparative fluorescence two-dimensional gel electrophoresis (hCoFGE) for improved spot coordinate detection Electrophoresis. 2014;35:1118–1121. PubMed

pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes