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Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique for studying the role of FerB protein in oxidative stress in Paracoccus denitrificans

. 2015 Sep ; 4 () : 390-4. [epub] 20150706

Status PubMed-not-MEDLINE Language English Country Netherlands Media electronic-ecollection

Document type Journal Article

Links

PubMed 26306309
PubMed Central PMC4534580
DOI 10.1016/j.dib.2015.06.015
PII: S2352-3409(15)00110-9
Knihovny.cz E-resources

3DLC protein- and peptide-fractionation technique combined with iTRAQ-peptide labeling and Orbitrap mass spectrometry was employed to quantitate Paracoccus dentirificans total proteome with maximal coverage. This resulted in identification of 24,948 peptides representing 2627 proteins (FDR<0.01) in P. dentirificans wild type and ferB mutant strains grown in the presence or absence of methyl viologen as an oxidative stressor. The data were generated for assessment of FerB protein role in oxidative stress as published by Pernikářová et al.; proteomic responses to a methyl viologen-induced oxidative stress in the wild type and FerB mutant strains of P. denitrificans, J. Proteomics 2015;125:68-75. Dataset is supplied in the article.

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Pernikarova V., Sedlacek V., Potesil D., Struharova I., Zdrahal Z., Kucera I., Bouchal P. Proteomic responses to a methyl viologen-induced oxidative stress in the wild type and FerB mutant strains of Paracoccus denitrificans. J. Proteomics. 2015;125:68–75. PubMed

Bouchal P., Precechtelova P., Zdrahal Z., Kucera I. Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide. Proteomics. 2004;4:2662–2671. PubMed

Bouchal P., Struharova I., Budinska E., Sedo O., Vyhlidalova T., Zdrahal Z. Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach. Biochim. Biophys. Acta. 2010;1804:1350–1358. PubMed

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Wiśniewski J.R., Zougman A., Nagaraj N., Mann M. Universal sample preparation method for proteome analysis. Nat. Methods. 2009;6:359–362. PubMed

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