Evaluation of baicalein, chitosan and usnic acid effect on Candida parapsilosis and Candida krusei biofilm using a Cellavista device
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26362224
DOI
10.1016/j.mimet.2015.09.002
PII: S0167-7012(15)30059-2
Knihovny.cz E-resources
- Keywords
- Baicalein, Biofilm, Candida krusei, Candida parapsilosis, Cellavista, Chitosan, Eradication, Inhibition,
- MeSH
- Amphotericin B pharmacology MeSH
- Anti-Infective Agents pharmacology MeSH
- Benzofurans pharmacology MeSH
- Biofilms drug effects MeSH
- Candida drug effects physiology MeSH
- Chitosan pharmacology MeSH
- Flavanones pharmacology MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Microscopy methods MeSH
- Image Processing, Computer-Assisted MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amphotericin B MeSH
- Anti-Infective Agents MeSH
- baicalein MeSH Browser
- Benzofurans MeSH
- Chitosan MeSH
- Flavanones MeSH
- usnic acid MeSH Browser
Biofilms are often the cause of chronic human infections and contaminate industrial or medical equipment. The traditional approach has been to use increasing concentrations of antibiotics, but microorganisms rapidly develop multiresistance to them. Therefore, we investigated the use of natural substances as an alternative solution. The quantification of the biofilms based on the colonized areas was measured using a Cellavista automatic microscope equipped with image analysis software. Using the Cellavista device brings new possibilities for qualification and quantification of sessile cells. In our study, this feature was documented by exploring the antifungal/anti-biofilm activity of amphotericin B, baicalein, chitosan and usnic acid against yeast biofilm formation. The influence of these substances on the formation and eradication of opportunistic pathogenic yeasts Candida parapsilosis and Candida krusei biofilms was studied in 96-well polystyrene microtiter plates. While amphotericin B was not very efficient, the use of baicalein and chitosan, even in minimum inhibitory concentrations, was found to rapidly decrease the colonized areas in the wells. The usnic acid did not display any significant antibiofilm properties even at concentration 300μgml(-1). Our results propose that Cellavista is a promising tool for the study of yeast biofilm formation and the effects of antimicrobial agents.
References provided by Crossref.org
Composition and Biological Activity of Vitis vinifera Winter Cane Extract on Candida Biofilm
Effect of resveratrol and Regrapex-R-forte on Trichosporon cutaneum biofilm
Resveratrol, pterostilbene, and baicalein: plant-derived anti-biofilm agents