Size matters: Associations between the androgen receptor CAG repeat length and the intrafollicular hormone milieu
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26404660
DOI
10.1016/j.mce.2015.09.015
PII: S0303-7207(15)30081-2
Knihovny.cz E-zdroje
- Klíčová slova
- Androgen levels, Androgen receptor, CAG repeats, Follicular fluid, Human small antral follicles,
- MeSH
- androgenní receptory genetika MeSH
- aromatasa genetika MeSH
- dospělí MeSH
- expanze trinukleotidových repetic * MeSH
- folikulární tekutina cytologie metabolismus MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- pohlavní hormony genetika metabolismus MeSH
- receptory LH genetika MeSH
- stanovení celkové genové exprese metody MeSH
- testosteron metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- androgenní receptory MeSH
- AR protein, human MeSH Prohlížeč
- aromatasa MeSH
- CYP19A1 protein, human MeSH Prohlížeč
- pohlavní hormony MeSH
- receptory LH MeSH
- testosteron MeSH
Granulosa cell (GC) expressed androgen receptors (AR) and intrafollicular androgens are central to fertility. The transactivating domain of the AR contains a polymorphic CAG repeat sequence, which is linked to the transcriptional activity of AR and may influence the GC function. This study aims to evaluate the effects of the AR CAG repeat length on the intrafollicular hormone profiles, and the gene expression profiles of GC from human small antral follicles. In total, 190 small antral follicles (3-11 mm in diameter) were collected from 58 women undergoing ovarian cryopreservation for fertility preservation. The biallelic mean of the CAG repeat lengths were calculated for each woman, and grouped in three groups: Long CAG repeats (23-26 mean CAG); medium CAG repeats (20.5-22.5 mean CAG) and short CAG repeats (17.5-20.0 mean CAG). The following parameters were measured: follicle diameter, intrafollicular levels of Anti-Müllerian Hormone (AMH), progesterone, oestradiol, testosterone and androstenedione, and GC gene expression levels of FSHR, LHR, AR, CYP19A1, and AMH. The long CAG repeat lengths were associated with significantly decreased testosterone levels, as compared to medium CAG repeats (P = 0.05) and short CAG repeats (P = 0.003). Furthermore, in follicles 3-6 mm in diameter, the long CAG repeats were associated with significantly increased LHR and CYP19A1 gene expression levels compared to short CAG repeat lengths (P = 0.004 and P = 0.04 respectively), and significantly increased LHR expression compared to medium CAG repeat lengths (P = 0.03). In conclusion, long CAG repeat lengths in the AR were associated to significant attenuated levels of androgens and an increased conversion of testosterone into oestradiol, in human small antral follicles.
Department of Biomedicine University of Aarhus Aarhus Denmark
Laboratory of Reproductive Biology Rigshospitalet Copenhagen Denmark
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