High levels of WNT-5A in human glioma correlate with increased presence of tumor-associated microglia/monocytes
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26511503
DOI
10.1016/j.yexcr.2015.10.022
PII: S0014-4827(15)30133-6
Knihovny.cz E-resources
- Keywords
- Glioma, Major histocompatibility complex II, Microglia, Tumor microenvironment, WNT-5A,
- MeSH
- Tissue Array Analysis MeSH
- Glioma metabolism pathology MeSH
- Humans MeSH
- Microglia metabolism pathology MeSH
- Monocytes metabolism pathology MeSH
- Wnt-5a Protein MeSH
- Wnt Proteins biosynthesis genetics metabolism MeSH
- Proto-Oncogene Proteins biosynthesis genetics metabolism MeSH
- Computational Biology MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Wnt-5a Protein MeSH
- Wnt Proteins MeSH
- Proto-Oncogene Proteins MeSH
- WNT5A protein, human MeSH Browser
Malignant gliomas are among the most severe types of cancer, and the most common primary brain tumors. Treatment options are limited and the prognosis is poor. WNT-5A, a member of the WNT family of lipoglycoproteins, plays a role in oncogenesis and tumor progression in various cancers, whereas the role of WNT-5A in glioma remains obscure. Based on the role of WNT-5A as an oncogene, its potential to regulate microglia cells and the glioma-promoting capacities of microglia cells, we hypothesize that WNT-5A has a role in regulation of immune functions in glioma. We investigated WNT-5A expression by in silico analysis of the cancer genome atlas (TCGA) transcript profiling of human glioblastoma samples and immunohistochemistry experiments of human glioma tissue microarrays (TMA). Our results reveal higher WNT-5A protein levels and mRNA expression in a subgroup of gliomas (WNT-5A(high)) compared to non-malignant control brain tissue. Furthermore, we show a significant correlation between WNT-5A in the tumor and presence of major histocompatibility complex Class II-positive microglia/monocytes. Our data pinpoint a positive correlation between WNT-5A and a proinflammatory signature in glioma. We identify increased presence of microglia/monocytes as an important aspect in the inflammatory transformation suggesting a novel role for WNT-5A in human glioma.
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