Distribution of pathogenicity island markers in commensal and uropathogenic Escherichia coli isolates
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
26563230
DOI
10.1007/s12223-015-0433-8
PII: 10.1007/s12223-015-0433-8
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Drug Resistance, Bacterial MeSH
- Genetic Markers * MeSH
- Genome, Bacterial * MeSH
- Genomic Islands * MeSH
- Escherichia coli Infections microbiology MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Case-Control Studies MeSH
- Symbiosis MeSH
- Uropathogenic Escherichia coli drug effects genetics pathogenicity MeSH
- Virulence genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Genetic Markers * MeSH
Uropathogenic Escherichia coli (UPEC) isolates contain large genomic segments, termed pathogenicity islands (PAIs), that contribute to their virulence. A total of 150 UPEC and 50 commensal E. coli isolates from outpatients were investigated for antimicrobial susceptibility and the presence of eight PAI markers. One hundred ninety (95 %) isolates were resistant to one or more antimicrobial agents. The most frequent resistance found against amoxicillin (68 %), amoxicillin/clavulanic acid (55 %), aztreonam (50 %), trimethoprim/sulfamethoxazole (46 %) and tetracycline (43.5 %). Antimicrobial resistance among UPEC isolates was higher than that of commensals. PAI markers were detected in substantial percentage of commensal (88 %) and UPEC isolates (98.6 %) (P > 0.05). The most prevalent PAI marker among UPEC and commensal isolates was PAI IV536 (98.7 % UPEC vs. 84 % commensal). We found a high number of PAI markers such as PAI ICFT073, PAI IICFT073, PAI I536, PAI II536, PAI III536 and PAI IIJ96 significantly associated with UPEC. PAI III536 (21.3 %) and PAI IIJ96 (8 %) were detected only in the uropathogenic isolates. Several different combinations of PAIs were found among UPEC isolates. Comparison of PAIs among UPEC and commensal isolates showed that many UPEC isolates (79.3 %) carried two or more PAI markers, while 6 % of commensals had two PAI markers (P < 0.05). The most frequent combinations of PAI markers in UPEC isolates were PAI IV536 + PAI IICFT073 (18 %) and PAI IV536 + PAI ICFT073 + PAI IICFT073 (18 %). These results indicate that PAI markers are widespread among commensal and UPEC isolates and these commensal isolates may be reservoirs for transmission of these markers.
See more in PubMed
Cell Microbiol. 2006 Nov;8(11):1707-19 PubMed
Ann Lab Med. 2014 Mar;34(2):139-44 PubMed
Braz J Microbiol. 2011 Oct;42(4):1308-13 PubMed
Pathogens. 2014 Mar 31;3(2):238-48 PubMed
Braz J Med Biol Res. 2008 Oct;41(10):877-83 PubMed
BMC Bioinformatics. 2005 Jul 21;6:184 PubMed
Curr Opin Microbiol. 2009 Oct;12(5):476-81 PubMed
Nat Rev Microbiol. 2010 Aug;8(8):552-63 PubMed
Appl Environ Microbiol. 2010 Oct;76(19):6698-700 PubMed
Ann Clin Microbiol Antimicrob. 2009 Dec 08;8:34 PubMed
Infect Immun. 2002 Nov;70(11):6365-72 PubMed
Pathogens. 2014 Jan 13;3(1):36-56 PubMed
Appl Environ Microbiol. 2013 Jun;79(11):3406-12 PubMed
Med Sci Monit. 2013 May 01;19:317-26 PubMed
Clin Microbiol Rev. 2004 Jan;17(1):14-56 PubMed
Jundishapur J Microbiol. 2015 Feb 07;8(2):e17514 PubMed
Biomed Res Int. 2014;2014:465054 PubMed
Arch Iran Med. 2012 May;15(5):312-6 PubMed
Clin Microbiol Infect. 2006 Sep;12(9):880-6 PubMed
Appl Environ Microbiol. 2011 Apr;77(7):2303-8 PubMed
FEMS Microbiol Lett. 2006 Jan;254(1):75-80 PubMed
J Bacteriol. 2004 May;186(10):3086-96 PubMed
Microb Pathog. 1990 Mar;8(3):213-25 PubMed
Genet Mol Res. 2011 Oct 31;10(4):4114-25 PubMed
