LC-MS/MS method for the determination of haemanthamine in rat plasma, bile and urine and its application to a pilot pharmacokinetic study
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Validation Study
PubMed
26577707
DOI
10.1002/bmc.3653
Knihovny.cz E-resources
- Keywords
- LC-MS/MS assay, ambelline, haemanthamine, pharmacokinetics,
- MeSH
- Amaryllidaceae Alkaloids blood pharmacokinetics urine MeSH
- Phenanthridines blood pharmacokinetics urine MeSH
- Rats MeSH
- Limit of Detection MeSH
- Pilot Projects MeSH
- Reproducibility of Results MeSH
- Tandem Mass Spectrometry methods MeSH
- Bile metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Validation Study MeSH
- Names of Substances
- Amaryllidaceae Alkaloids MeSH
- Phenanthridines MeSH
- hemanthamine MeSH Browser
Evidence gathered in various studies points to the fact that haemanthamine, an isoquinoline alkaloid, has multiple medicinally interesting characteristics, including antitumor, antileukemic, antioxidant, antiviral, anticonvulsant and antimalarial activity. This work presents, for the first time, a universal LC-MS/MS method for analysis of haemanthamine in plasma, bile and urine which has been verified in a pilot pharmacokinetic experiment on rats. Chromatographic separation was performed on a pentafluorophenyl core-shell column in gradient elution mode with a mobile phase consisting of acetonitrile-methanol-ammonium formate buffer. A sample preparation based on liquid-liquid extraction with methyl tert-butyl ether was employed with ambelline used as an internal standard. Quantification was performed using LC-MS-ESI(+) in Selected Reaction Monitoring mode. The method was validated according to the European Medicines Agency guideline in a concentration range of 0.1-10 μmol/L in plasma, bile and urine. The concentration-time profiles of haemanthamine in plasma, bile and urine after a single i.v. bolus of 10 mg/kg have been described for the first time. The presented study addresses the lack of information on haemanthamine pharmacokinetics and also introduces a new universal method of haemanthamine analysis in complex biological matrices. Copyright © 2015 John Wiley & Sons, Ltd.
References provided by Crossref.org
The Genus Nerine Herb. (Amaryllidaceae): Ethnobotany, Phytochemistry, and Biological Activity
