Synthesis of silver nanoparticles on the basis of low and high molar mass exopolysaccharides of Bradyrhizobium japonicum 36 and its antimicrobial activity against some pathogens
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26603749
DOI
10.1007/s12223-015-0436-5
PII: 10.1007/s12223-015-0436-5
Knihovny.cz E-zdroje
- MeSH
- antiinfekční látky metabolismus farmakologie MeSH
- bakteriální polysacharidy metabolismus MeSH
- Bradyrhizobium metabolismus MeSH
- Candida albicans účinky léků MeSH
- difrakce rentgenového záření MeSH
- Escherichia coli účinky léků MeSH
- mikrobiální testy citlivosti MeSH
- nanočástice chemie metabolismus ultrastruktura MeSH
- oxidace-redukce MeSH
- spektrofotometrie MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- Staphylococcus aureus MeSH
- stříbro metabolismus farmakologie MeSH
- transmisní elektronová mikroskopie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antiinfekční látky MeSH
- bakteriální polysacharidy MeSH
- stříbro MeSH
Silver nanoparticles (SNPs) were synthesized on the basis of exopolysaccharides (low and high molar mass) of diazotrophic Bradyrhizobium japonicum 36 strain. The synthesis of SNPs was carried out by direct reduction of silver nitrate with ethanol-insoluble (high molar mass, HMW) and ethanol-soluble (low molar mass, LMW) fractions of exopolysaccharides (EPS), produced by diazotrophic strain B. japonicum 36. SNPs were characterized using UV-vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). SNPs synthesized on the basis of LMW EPS absorbed radiation in the visible regions of 420 nm, whereas SNPs based on the HMW EPS have a wavelength maximum at 450 nm because of the strong SPR transition. Moreover, the antibacterial and antifungal activities of the SNPs were examined in vitro against Escherichia coli, Staphylococcus aureus, and Candida albicans. SNPs synthesized on the basis of LMW EPS were active than those synthesized on the basis of HMW EPS. Besides, UV-visible spectroscopic evaluation confirmed that SNPs synthesized on the basis of LMW EPS were far more stable than those obtained on the basis of HMW EPS.
Department of Biology Samarkand State University University Boulevard 15 Samarkand 703004 Uzbekistan
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