Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Mercury, microbial community, mobility, rhizobox, speciation,
- MeSH
- Bacteria metabolism MeSH
- Biodegradation, Environmental MeSH
- Chromatography, Gas MeSH
- Spectrometry, Fluorescence MeSH
- Soil Pollutants analysis chemistry MeSH
- Methylmercury Compounds analysis chemistry MeSH
- Environmental Monitoring methods MeSH
- Soil Microbiology * MeSH
- Mercury analysis chemistry MeSH
- Mercury Compounds analysis chemistry MeSH
- Spectrophotometry, Atomic MeSH
- Environmental Pollution analysis MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Soil Pollutants MeSH
- Methylmercury Compounds MeSH
- Mercury MeSH
- Mercury Compounds MeSH
The precise characterization of the behavior of individual microorganisms in the presence of increased mercury contents in soil is necessary for better elucidation of the fate of mercury in the soil environment. In our investigation, resistant bacterial strains isolated from two mercury contaminated soils, represented by Paenibacillus alginolyticus, Burkholderia glathei, Burkholderia sp., and Pseudomonas sp., were used. Two differently contaminated soils (0.5 and 7 mg kg(-1) total mercury) were chosen. Preliminary soil analysis showed the presence of methylmercury and phenylmercury with the higher soil mercury level. Modified rhizobox experiments were performed to assess the ability of mercury accumulating strains to deplete the mobile and mobilizable mercury portions in the soil by modification; microbial agar cultures were used rather than the plant root zone. A sequential extraction procedure was performed to release the following mercury fractions: water soluble, extracted in acidic conditions, bound to humic substances, elemental, and bound to complexes, HgS and residual. Inductively coupled plasma mass spectrometry (ICP-MS) and a single-purpose atomic absorption spectrometer (AMA-254) were applied for mercury determination in the samples and extracts. Gas chromatography coupled to atomic fluorescence spectrometry (GC-AFS) was used for the determination of organomercury compounds. The analysis of the microbial community at the end of the experiment showed a 42% abundance of Paenibacillus sp. followed by Acetivibrio sp., Brevibacillus sp., Cohnella sp., Lysinibacillus sp., and Clostridium sp. not exceeding 2% abundance. The results suggest importance of Paenibacillus sp. in Hg transformation processes. This genus should be tested for potential bioremediation use in further research.
b Institute of Microbiology Vídeňská Prague Czech Republic
c Atomic Absorption Spectrometry Laboratory Institute of Chemical Technology Prague Czech Republic
Department of Chemistry Faculty of Science Masaryk University Brno Czech Republic
e Institute of Analytical Chemistry Academy of Sciences of the Czech Republic Brno Czech Republic
f Department of Public Health Faculty of Medicine Masaryk University Brno Czech Republic
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