Detekce DNA Neisseria meningitidis, Haemophilus influenzae a Streptococcus pneumoniae v klinickém materiálu metodou real-time PCR
[Real-time PCR detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae DNA in clinical specimens]
Language Czech Country Czech Republic Media print
Document type Evaluation Study, Journal Article
PubMed
26795226
PII: 57186
- Keywords
- Haemophilus influenzae, and Streptococcus pneumoniae - rt-PCR -detection of the agents of bacterial meningitis and sepsis., rt-PCR detection of Neisseria meningitidis,
- MeSH
- Adult MeSH
- Haemophilus influenzae classification genetics isolation & purification MeSH
- Haemophilus Infections diagnosis microbiology MeSH
- Real-Time Polymerase Chain Reaction methods MeSH
- Middle Aged MeSH
- Humans MeSH
- Meningitis, Bacterial diagnosis microbiology MeSH
- Neisseria meningitidis classification genetics isolation & purification MeSH
- Pneumococcal Infections diagnosis microbiology MeSH
- Sensitivity and Specificity MeSH
- Streptococcus pneumoniae classification genetics isolation & purification MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Geographicals
- Czech Republic MeSH
- United States MeSH
The study aim was to implement a molecular real-time polymerase chain reaction (PCR) assay recommended by the CDC (Centers for Disease Control and Prevention) for the detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical (culture negative) specimens from patients with suspected invasive bacterial disease. Clinical specimens are referred to the National Reference Laboratory (NRL) for Meningococcal Infections, Unit for Airborne Bacterial Infections, Centre for Epidemiology and Microbiology, National Institute of Public Health from various regions of the Czech Republic. Clinical specimens are, in particular, cerebrospinal fluid, anti-coagulated blood or serum and, exceptionally, post-mortem specimens. The NRL has implemented molecular diagnosis of these bacterial pathogens involved in meningitis and sepsis from clinical specimens since 1999. The first diagnostic method was semi-nested PCR followed by electrophoretic analysis. In 2014, a molecular qualitative real-time PCR assay was implemented.
