Differentiation of Vascular Stem Cells Contributes to Ectopic Calcification of Atherosclerotic Plaque
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26840742
DOI
10.1002/stem.2315
Knihovny.cz E-zdroje
- Klíčová slova
- Atherosclerosis, Chondrogenesis, Collagen scaffold, Endochondral ossification, In vivo, Mesenchymal stem cells, Pericytes, Vascular calcification, Vascular progenitor cells,
- MeSH
- apolipoproteiny E genetika MeSH
- aterosklerotický plát genetika patologie terapie MeSH
- ateroskleróza genetika patologie terapie MeSH
- buněčná diferenciace genetika MeSH
- cévy cytologie MeSH
- chondrogeneze genetika MeSH
- cytokiny metabolismus MeSH
- glykosaminoglykany metabolismus MeSH
- interleukin-6 metabolismus MeSH
- lidé MeSH
- mezenchymální kmenové buňky * MeSH
- myši MeSH
- osteogeneze genetika MeSH
- vaskulární kalcifikace genetika metabolismus patologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- apolipoproteiny E MeSH
- cytokiny MeSH
- glykosaminoglykany MeSH
- interleukin-6 MeSH
The cellular and molecular basis of vascular calcification (VC) in atherosclerosis is not fully understood. Here, we investigate role of resident/circulating progenitor cells in VC and contribution of inflammatory plaque environment to this process. Vessel-derived stem/progenitor cells (VSCs) and mesenchymal stem cells (MSCs) isolated from atherosclerotic ApoE(-/-) mice showed significantly more in vitro osteogenesis and chondrogenesis than cells generated from control C57BL/6 mice. To assess their ability to form bone in vivo, cells were primed chondrogenically or cultured in control medium on collagen glycosaminoglycan scaffolds in vitro prior to subcutaneous implantation in ApoE(-/-) and C57BL/6 mice using a crossover study design. Atherosclerotic ApoE(-/-) MSCs and VSCs formed bone when implanted in C57BL/6 mice. In ApoE(-/-) mice, these cells generated more mature bone than C57BL/6 cells. The atherosclerotic in vivo environment alone promoted bone formation by implanted C57BL/6 cells. Un-primed C57BL/6 VSCs were unable to form bone in either mouse strain. Treatment of ApoE(-/-) VSC chondrogenic cultures with interleukin (IL)-6 resulted in significantly increased glycosaminoglycan deposition and expression of characteristic chondrogenic genes at 21 days. In conclusion, resident vascular cells from atherosclerotic environment respond to the inflammatory milieu and undergo calcification. IL-6 may have a role in aberrant differentiation of VSCs contributing to vascular calcification in atherosclerosis.
Advanced Materials and Bioengineering Research Centre RCSI and TCD Dublin Ireland
Division of Cardiology Johns Hopkins University School of Medicine Baltimore Maryland USA
Regenerative Medicine Institute National University of Ireland Galway Galway Ireland
Trinity Centre for Bioengineering Trinity College Dublin Dublin 2 Ireland
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