Technical note: Efficient removal of a PCR inhibitory agent (vivianite) found on excavated bones
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26874049
DOI
10.1016/j.forsciint.2015.12.043
PII: S0379-0738(15)00544-7
Knihovny.cz E-resources
- Keywords
- DNA extraction, PCR inhibitors, Vivianite,
- MeSH
- DNA Fingerprinting MeSH
- DNA isolation & purification MeSH
- Phosphates adverse effects MeSH
- Humans MeSH
- Specimen Handling instrumentation methods MeSH
- Burial MeSH
- Polymerase Chain Reaction * MeSH
- Forensic Anthropology instrumentation methods MeSH
- Ferrous Compounds adverse effects MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA MeSH
- ferrous phosphate MeSH Browser
- Phosphates MeSH
- Ferrous Compounds MeSH
During a rescue excavation in October 2011, archaeologists discovered a mass grave with 10 individuals. The skeletons should belong to victims of the battle of Reichenberg between the Austrian and Prussian armies on April 21, 1757. Several bones of the skeletons were covered with a blue colored encrustation. Initial DNA analysis failed due to strong inhibition. Chemical analysis of the bluish encrustation indicated the presence of the iron phosphate mineral vivianite (Fe3(PO4)2·(H2O)8). This technical note describes a novel procedure for the removal of this inhibitory substance.
Forensic DNA Service Budinova 2 180 81 Prague 8 Czech Republic
Institute of Archaeology of the Academy of Sciences Prague Czech Republic
Institute of Legal Medicine Bulovka Hospital Prague Czech Republic
North Bohemian Museum Liberec Czech Republic
University of Chemistry and Technology Prague Prague Czech Republic
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