Rat intra-hippocampal NMDA infusion induces cell-specific damage and changes in expression of NMDA and GABAA receptor subunits
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26930443
DOI
10.1016/j.neuropharm.2016.02.035
PII: S0028-3908(16)30070-3
Knihovny.cz E-zdroje
- Klíčová slova
- Carousel maze, Excitotoxicity, GABA A receptor, Hippocampus, Interneurons, NMDA receptor, Neurodegeneration, Neuroinflammation, Spatial learning,
- MeSH
- bludiště - učení účinky léků fyziologie MeSH
- degenerace nervu diagnostické zobrazování metabolismus patologie MeSH
- funkční lateralita MeSH
- hipokampus diagnostické zobrazování účinky léků metabolismus patologie MeSH
- modely nemocí na zvířatech MeSH
- N-methylaspartát aplikace a dávkování toxicita MeSH
- neurodegenerativní nemoci diagnostické zobrazování metabolismus patologie MeSH
- neuroimunomodulace fyziologie MeSH
- neurony účinky léků metabolismus patologie MeSH
- potkani Long-Evans MeSH
- receptory GABA-A metabolismus MeSH
- receptory N-methyl-D-aspartátu metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- Gabra5 protein, rat MeSH Prohlížeč
- N-methylaspartát MeSH
- NR1 NMDA receptor MeSH Prohlížeč
- NR2B NMDA receptor MeSH Prohlížeč
- receptory GABA-A MeSH
- receptory N-methyl-D-aspartátu MeSH
Excessive stimulation of NMDA receptors with glutamate or other potent agonists such as NMDA leads to excitotoxicity and neural injury. In this study, we aimed to provide insight into an animal model of brain excitotoxic damage; single unilateral infusion of NMDA at mild dose into the hippocampal formation. NMDA infusion induced chronic, focal neurodegeneration in the proximity of the injection site. The lesion was accompanied by severe and progressive neuroinflammation and affected preferentially principal neurons while sparing GABAergic interneurons. Furthermore, the unilateral lesion did not cause significant impairment of spatial learning abilities. Finally, GluN1 and GluN2B subunits of NMDA receptor were significantly upregulated up to 3 days after the NMDA infusion, while GABAA α5 subunit was downregulated at 30 days after the lesion. Taken together, a single infusion of NMDA into the hippocampal formation represents an animal model of excitotoxicity-induced chronic neurodegeneration of principal neurons accompanied by severe neuroinflammation and subunit specific changes in NMDA and GABAA receptors.
Citace poskytuje Crossref.org
