Tissue microRNAs as predictive markers for gastric cancer patients undergoing palliative chemotherapy
Language English Country Greece Media print-electronic
Document type Journal Article
PubMed
27081844
DOI
10.3892/ijo.2016.3484
Knihovny.cz E-resources
- MeSH
- Cisplatin administration & dosage pharmacology MeSH
- Fluorouracil administration & dosage pharmacology MeSH
- Humans MeSH
- MicroRNAs genetics MeSH
- Biomarkers, Tumor genetics MeSH
- Stomach Neoplasms drug therapy genetics MeSH
- Palliative Care MeSH
- Prognosis MeSH
- Disease Progression MeSH
- Antineoplastic Combined Chemotherapy Protocols administration & dosage pharmacology MeSH
- Gene Expression Regulation, Neoplastic drug effects MeSH
- Gene Expression Profiling MeSH
- Treatment Outcome MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Cisplatin MeSH
- Fluorouracil MeSH
- MicroRNAs MeSH
- Biomarkers, Tumor MeSH
MicroRNAs have the potential to become valuable predictive markers for gastric cancer. Samples of biopsy tissue, routinely taken from gastric cancer patients undergoing palliative chemotherapy, constitute suitable material for microRNA profiling with the aim of predicting the effect of chemotherapy. Our study group consisted of 54 patients, all of whom underwent palliative chemotherapy based on 5-fluorouracil (5-FU) or 5-FU in combination with platinum derivatives between 2000 and 2013. The expression of 29 selected microRNAs and genes BRCA1, ERCC1, RRM1 and TS, in gastric cancer tissue macrodissected from FFPE tissue samples, was measured by quantitative RT-PCR. The relationship between gene expression levels and time to progression (TTP) and overall survival (OS) was analysed. From the set of the 29 microRNAs of interest, we found high expression of miR-150, miR-342-3p, miR-181b, miR-221, miR-224 and low levels of miR-520h relate to shorter TTP. High levels of miR-150, miR-192, miR-224, miR-375 and miR-342-3p related to shorter OS. In routinely available FFPE tissue samples, we found 6 miRNAs with a relation to TTP, which may serve as predictors of the effectiveness of palliative treatment in gastric cancer patients. These miRNAs could also help in deciding whether to indicate palliative chemotherapy.
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