Acute temperature effects on function of the chick embryonic heart
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27083765
DOI
10.1111/apha.12691
Knihovny.cz E-resources
- Keywords
- arrhythmias, calcium imaging, chick embryo, conduction block, heart development, optical mapping,
- MeSH
- Atrioventricular Block physiopathology MeSH
- Electric Stimulation MeSH
- Cardiac Imaging Techniques MeSH
- Cardiac Pacing, Artificial MeSH
- Chick Embryo MeSH
- Myocardium metabolism MeSH
- Heart Conduction System physiopathology MeSH
- Heart diagnostic imaging physiology physiopathology MeSH
- Arrhythmias, Cardiac physiopathology MeSH
- Heart Rate physiology MeSH
- Tachycardia physiopathology MeSH
- Temperature * MeSH
- Calcium metabolism MeSH
- Calcium Signaling physiology MeSH
- Animals MeSH
- Check Tag
- Chick Embryo MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Calcium MeSH
AIM: We analysed the effects of acute temperature change on the beating rate, conduction properties and calcium transients in the chick embryonic heart in vitro and in ovo. METHODS: The effects of temperature change (34, 37 and 40 °C) on calcium dynamics in isolated ED4 chick hearts in vitro were investigated by high-speed calcium optical imaging. For comparison and validation of in vitro measurements, experiments were also performed in ovo using videomicroscopy. Artificial stimulation experiments were performed in vitro and in ovo to uncover conduction limits of heart segments. RESULTS: Decrease in temperature from 37 to 34 °C in vitro led to a 22% drop in heart rate and unchanged amplitude of Ca(2+) transients, compared to a 25% heart rate decrease in ovo. Increase in temperature from 37 to 40 °C in vitro and in ovo led to 20 and 23% increases in heart rate, respectively, and a significant decrease in amplitude of Ca(2+) transients (atrium -35%, ventricle -38%). We observed a wide spectrum of arrhythmias in vitro, of which the most common was atrioventricular (AV) block (57%). There was variability of AV block locations. Pacing experiments in vitro and in ovo suggested that the AV blocks were likely caused by relative tissue hypoxia and not by the tachycardia itself. CONCLUSION: The pacemaker and AV canal are the most temperature-sensitive segments of the embryonic heart. We suggest that the critical point for conduction is the connection of the ventricular trabecular network to the AV canal.
1st Faculty of Medicine Institute of Anatomy Charles University Prague Czech Republic
Czech Academy of Sciences Institute of Physiology Prague Czech Republic
References provided by Crossref.org
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