Isolation and identification of Arcobacter species from environmental and drinking water samples
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
27106697
DOI
10.1007/s12223-016-0460-0
PII: 10.1007/s12223-016-0460-0
Knihovny.cz E-resources
- MeSH
- Aerobiosis MeSH
- Arcobacter classification genetics growth & development isolation & purification MeSH
- Sodium Chloride metabolism MeSH
- DNA, Bacterial chemistry genetics MeSH
- Culture Media chemistry MeSH
- Water Microbiology * MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Bacterial Typing Techniques MeSH
- Temperature MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Turkey MeSH
- Names of Substances
- Sodium Chloride MeSH
- DNA, Bacterial MeSH
- Culture Media MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
Water plays an important role in the transmission of Arcobacter spp. to animals and humans. The aim of this study was to isolate and characterize Arcobacter spp. from 115 different water samples (66 sewage, 25 rivers, 16 spring water, and 8 drinking water) in Izmir, Turkey. In total, 41 samples (35.7 %) were found positive for Arcobacter spp. by the genus-specific PCR. Arcobacter butzleri was detected in 39 out of 115 samples (33.9 %) including 24 sewage, 13 rivers, and 2 spring water. The remaining Arcobacter spp. (n = 2) isolates could not be identified by m-PCR and 16S rRNA gene sequencing. Based on the phenotypic characterization, most of the Arcobacter species (87.8 %) indicated weak catalase activity. In addition, there were differences in phenotypic patterns among isolated species during growth at 37 °C under microaerobic and aerobic conditions, in the presence of 2 % (39/41) and 3.5 % (32/41) NaCl and 0.04 % TTC (39/41) and on MacConkey agar (38/41). The results of this study indicated that environmental water samples are common sources for Arcobacter spp. Therefore, effective control measures should be taken to protect human health.
Department of Bioengineering and Biotechnology Izmir Institute of Technology Urla 35430 Izmir Turkey
Department of Food Engineering Izmir Institute of Technology Urla 35430 Izmir Turkey
Department of Medical Microbiology Faculty of Medicine Sifa University 35100 Izmir Turkey
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