Modified isolation method of Arcobacter spp. from different environmental and food samples
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
25912846
DOI
10.1007/s12223-015-0395-x
PII: 10.1007/s12223-015-0395-x
Knihovny.cz E-zdroje
- MeSH
- Arcobacter genetika růst a vývoj izolace a purifikace metabolismus MeSH
- kultivační média metabolismus MeSH
- odpadní vody mikrobiologie MeSH
- počet mikrobiálních kolonií přístrojové vybavení metody MeSH
- potravinářská mikrobiologie * MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- kultivační média MeSH
- odpadní vody MeSH
This study provides information on the occurrence of Arcobacter in several types of water and food products of animal origin in the Czech Republic. We processed 190 samples using the modified method, and the occurrence of Arcobacter spp. was confirmed in 36.8 % of these. This total incidence consisted of Arcobacter butzleri (27.3 %), Arcobacter cryaerophilus (8.4 %) and Arcobacter skirrowii (1.1 %). We newly described the common presence of Arcobacter spp. in sewage water in the Czech Republic that is released into waterways after processing in water treatment plants (86.7 %). All the acquired isolates were subject to detailed confirmation with subsequent species classification using multiplex PCR and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In this study, we used a modification of a method using passive filtration of an enriched sample, which could be suitable for the isolation of Arcobacter, especially in combination with Campylobacter selective agar chromogenic medium. Our studies have shown this agar to be quite suited to the isolation of Arcobacter and that it can be an appropriate instrument for accelerating culture diagnostics.
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Isolation and identification of Arcobacter species from environmental and drinking water samples