Hospital-acquired pneumonia (HAP) is one of the most serious complications in patients staying in intensive care units. This multicenter study of Czech patients with HAP aimed at assessing the clonality of bacterial pathogens causing the condition. Bacterial isolates were compared using pulsed-field gel electrophoresis. Included in this study were 330 patients hospitalized between May 1, 2013 and December 31, 2014 at departments of anesthesiology and intensive care medicine of four big hospitals in the Czech Republic. A total of 531 bacterial isolates were obtained, of which 267 were classified as etiological agents causing HAP. Similarity or identity was assessed in 231 bacterial isolates most frequently obtained from HAP patients. Over the study period, no significant clonal spread was noted. Most isolates were unique strains, and the included HAP cases may therefore be characterized as mostly endogenous. Yet there were differences in species and potential identical isolates between the participating centers. In three hospitals, Gram-negative bacteria (Enterobacteriaceae and Pseudomonas aeruginosa) prevailed as etiological agents, and Staphylococcus aureus was most prevalent in the fourth center.

Department of Microbiology Faculty of Medicine and Dentistry Palacký University Olomouc Hněvotínská 3 77515 Olomouc Czech Republic

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Klin Mikrobiol Infekc Lek. 2011 Aug;17(4):135-40 PubMed

Intensive Care Med. 2001 Aug;27(8):1263-8 PubMed

J Clin Microbiol. 1996 Dec;34(12 ):2914-20 PubMed

JAMA. 1995 Aug 23-30;274(8):639-44 PubMed

J Clin Microbiol. 1995 Sep;33(9):2233-9 PubMed

Chest. 2001 Feb;119(2 Suppl):373S-384S PubMed

Ann Clin Microbiol Antimicrob. 2004 Sep 15;3:17 PubMed

Eur J Intern Med. 2010 Oct;21(5):360-8 PubMed

J Hosp Infect. 2010 Dec;76(4):316-9 PubMed

New Microbiol. 2009 Jul;32(3):277-83 PubMed

Respir Care. 2005 Jun;50(6):813-36; discussion 836-8 PubMed

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2012 Dec;156(4):348-53 PubMed

Chest. 2000 May;117(5):1434-42 PubMed

Klin Mikrobiol Infekc Lek. 2005 Feb;11(1):20-4 PubMed

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